PXD068592

PXD068592 is an original dataset announced via ProteomeXchange.

Title	DNA methylation impacts soybean early development by modulate hormones and metabolic pathways
Description	Genomic DNA methylation patterns play a crucial role in the developmental processes of plants and mammals. In this study, we aimed to investigate the significant effects of epigenetic mechanisms on the development of soybean seedlings and metabolic pathways. Our analyses show that 5-azaC-treatment affects radicle development from two Days After Imbibition (DAI), as well as both shoot and root development. We examined the expression levels of key genes related to DNA methylation and demethylation pathways, such as DRM2, which encodes RNA-directed DNA Methylation (RdDM) pathway, SAM synthase responsible for methyl group donation, and ROS1, a DNA demethylase. In treated seedling roots, we observed an increase in DRM2 expression and a decrease in ROS1 expression. Additionally, 5-azaC treatment altered protein accumulation, indicating epigenetic control over stress response while inhibiting nitrogen assimilation, urea cycle, and glycolysis-related proteins. Furthermore, it influenced the levels of various phytohormones and metabolites crucial for seedling growth, such as ABA, IAA, ethylene, polyamines (PUT and Cad), and free amino acids, suggesting that epigenetic changes may shape soybean responses to pathogens, abiotic stress, and nutrient absorption. Our results assist in understanding how hypomethylation shapes soybean responses to pathogens, abiotic stress, and nutrient absorption crucial for seedling growth, suggesting that the plant's assimilation of carbon and nitrogen, along with hormone pathways, may be influenced by epigenetic changes.
HostingRepository	PRIDE
AnnounceDate	2026-05-05
AnnouncementXML	Submission_2026-05-05_11:54:31.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clicia Grativol
SpeciesList	scientific name: Glycine max; NCBI TaxID: NEWT:3847;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-19 13:13:57	ID requested
⏵ 1	2026-05-05 11:54:32	announced

Coelho FS, Miranda SS, Moraes JL, Hemerly AS, Ballesteros HGF, Santa-Catarina C, Dos Santos RC, de Almeida FA, Silveira V, Macedo A, Floh EIS, de Oliveira Alves Sena E, de Oliveira JG, Viccini LF, de Matos EM, Grativol C, DNA methylation impacts soybean early development by modulating hormones and metabolic pathways. Physiol Plant, 176(4):e14492(2024) [pubmed]

10.1111/ppl.14492;

submitter keyword: RdDM, 5-azaC, abiotic and biotic stress, carbon and nitrogen assimilation, Epigenetic control,Glycine max, polyamines metabolism

Clicia Grativol Gaspar de Matos
contact affiliation	Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia, Laboratório de Química e Função de Proteínas e Peptídeos, Prédio P5, Sala 228, Bairro Parque Califórnia, Campos dos Goytacazes, RS - CEP: 28013-602
contact email	cgrativol@uenf.br
lab head
Clicia Grativol
contact affiliation	Universidade Estadual do Norte Fluminense
contact email	cgrativol@uenf.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD068592

PRIDE project URI

• PRIDE
  1. PXD068592
     1. Label: PRIDE project
     2. Name: DNA methylation impacts soybean early development by modulate hormones and metabolic pathways