PXD068587

PXD068587 is an original dataset announced via ProteomeXchange.

Title	Profiling the RNA-Binding Proteome of KSHV-Infected Cells Uncovers a Viral Strategy for Hijacking Host Signaling via RNA-Dependent Interactions
Description	Viruses encode RNA-binding proteins (RBPs) to manipulate host processes and promote infection. However, due to limited genomic space, many viral proteins are multifunctional and may engage RNA through non-canonical domains, making their identification difficult using traditional sequence-based approaches. Kaposi’s sarcoma-associated herpesvirus (KSHV) is the etiological agent of many AIDS-associated cancers. Here, leveraging an unbiased proteomic approach we identified all RBPs in KSHV-infected primary effusion lymphoma (PEL) cells, uncovering 7 previously unrecognized KSHV-encoded RBPs. Among these is viral Interferon Regulatory Factor 1 (vIRF1), which we found is both necessary and sufficient to activate oncogenic RAS/MAPK signaling through an RNA-dependent interaction with host G3BP proteins. Importantly, a KSHV vIRF1 mutant lacking the ability to bind RNA exhibits a B cell-specific replication defect, highlighting the essential role of RNA interactions in viral gene regulation and pathogenesis. These findings reveal a novel strategy by which KSHV hijacks RNA-mediated signaling to support its lifecycle.
HostingRepository	PRIDE
AnnounceDate	2026-08-07
AnnouncementXML	Submission_2026-08-07_12:55:52.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-19 11:50:13	ID requested
⏵ 1	2026-08-07 12:55:53	announced

Dataset with its publication pending

submitter keyword: XRNAX, vIRF1 co-IP,KSHV, LC-MS/MS

John Karijolich
contact affiliation	Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center; Vanderbilt-Ingram Cancer Center; Vanderbilt Center for Immunobiology, and Vanderbilt Institute for Infection, Immunology, and Inflammation, Nashville, TN, 37232-2363, USA.,
contact email	john.karijolich@vumc.org
lab head
Yang Zhao
contact affiliation	Vanderbilt University Medical Center
contact email	yang.zhao.1@vumc.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068587
     1. Label: PRIDE project
     2. Name: Profiling the RNA-Binding Proteome of KSHV-Infected Cells Uncovers a Viral Strategy for Hijacking Host Signaling via RNA-Dependent Interactions