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PXD068567
PXD068567 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Immunopeptidome of Renal Cell Carcinoma (RCC) and Bladder Cancer (BC) Patient-Derived Tumor Organoids (PDTOs) |
| Description | Patient-Derived Tumor Organoids (PDTOs) have emerged as physiologically relevant systems, retaining genomic and phenotypic features of patient tumors and enabling drug response prediction. Here, we established a patient-specific pipeline to evaluate precision immunotherapy in Renal Cell Carcinoma (RCC) and Bladder Cancer (BC), two highly immunogenic malignancies. Tumor and adjacent benign tissues were used to generate PDTOs and Patient-Derived Cells (PDCs) for immunopeptidome profiling. Using our in-house microfluidic platform, PeptiCHIP for immunoaffinity purification followed by Ligandomics LC/MS analysis, we identified tumor-associated peptides presented by HLA-I molecules as potential targets for T cell–based immunotherapies. Their immunogenicity was later tested with peptide-expanded HLA-matched PBMCs, leading to the identification of peptides capable of eliciting antigen-specific CD8+ T cell activation and cytotoxicity against the patient’s tumor. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-24 |
| AnnouncementXML | Submission_2026-08-23_17:00:06.501.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rui Branca |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-19 05:41:59 | ID requested | |
| ⏵ 1 | 2026-08-23 17:00:07 | announced |
Publication List
| 10.1016/j.omton.2026.201247; |
| Antignani G, Feodoroff M, Chiaro J, Feola S, Russo S, Hamdan Hissaoui F, Fusciello M, D'Alessio F, Bottega P, Giannoula Y, Sakalauskaite M, Sandberg J, Kosonen M, Stigzelius V, Luck TJ, Ferrari V, Ciampi D, Haapala M, Branca RM, Partanen J, Koskela S, Rescigno M, Sikanen T, Lehti, ö J, Ndika J, Kari OK, Pieti, ä, inen VM, Gr, ö, nholm M, Cerullo V, Patient-derived tumor organoids for personalized cancer immunotherapy: An immunopeptidome-to-validation approach in RCC and BC. Mol Ther Oncol, 34(3):201247(2026) [pubmed] |
Keyword List
| submitter keyword: Ligandome, PDTOs, MHC, HLA-I, DDA,Immunopeptidomics, HLA, Immunopeptidome, Ligandomics, Patient-Derived Tumor Organoids, Renal Cell Carcinoma, RCC, TimsTOF SCP, MHC-I, Bladder Cancer |
Contact List
| Janne Lehtiö | |
|---|---|
| contact affiliation | Dept. Oncology-Pathology, Scilifelab, Karolinska Institutet, Sweden |
| contact email | janne.lehtio@ki.se |
| lab head | |
| Rui Branca | |
| contact affiliation | Clinical Proteomics Unit, Dep. of Oncology-Pathology |
| contact email | rui.mamede-branca@ki.se |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD068567 |
| PRIDE project URI |
Repository Record List
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