PXD068552

PXD068552 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicles isolated from fresh vs frozen melanoma tissues are similar in purity, protein composition, and tumor antigenicity
Description	Extracellular vesicles (EVs) are nano-sized particles released by cells, enriched in disease-related molecules, gaining attention as potential tumor biomarkers and useful tools for cancer immunotherapy. Tumor tissues are valuable sources of EVs, reflecting the tumor's molecular profile. This study investigates whether freezing tumor tissues affects the integrity and functionality of EVs and whether non-EV components contaminate them. We compared the purity and protein composition of EVs isolated from human metastatic melanoma tissue, analyzing both fresh and frozen samples. EVs were isolated using ultracentrifugation and an iodixanol density cushion, and characterized by transmission electron microscopy, nanoparticle tracking analysis, and mass spectrometry. Results showed no significant differences in purity, protein composition, or diagnostic potential between EVs from fresh and frozen tissues. Similar levels of melanoma-associated EV surface molecules were detected, with no signs of excess protein contamination in frozen tissue-derived EVs. Furthermore, we demonstrated that EVs from frozen mouse melanoma, when combined with synthetic bacterial vesicles, reduced tumor growth in melanoma-bearing mice to a similar extent as fresh tissue-derived EVs. This study supports the use of frozen tissues as a reliable EV source, as frozen tissue-derived EVs exhibit comparable purity, protein composition, diagnostic potential, and tumor antigenicity to those from fresh tissues.
HostingRepository	PRIDE
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-05-31_16:45:24.036.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Fuchs
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-19 02:56:44	ID requested
⏵ 1	2026-05-31 16:45:25	announced

10.1186/s12916-026-04923-8;

D Arrigo D, Park KS, L, ä, sser C, Sigalas GP, Symonds E, Urz, í O, Locatelli C, Olofsson Bagge R, L, ö, tvall J, Crescitelli R, Frozen melanoma tissues yield extracellular vesicles with preserved diagnostic and immunogenic properties. BMC Med, 24(1):(2026) [pubmed]

submitter keyword: TMT,Extracellular vesicles, melanoma, isolation, global quantitative proteomics

Rossella Crescitelli
contact affiliation	Sahlgrenska Center for Cancer Research, Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact email	rossella.crescitelli@gu.se
lab head
Johannes Fuchs
contact affiliation	Gothenburg University
contact email	johannes.fuchs@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD068552

PRIDE project URI

• PRIDE
  1. PXD068552
     1. Label: PRIDE project
     2. Name: Extracellular vesicles isolated from fresh vs frozen melanoma tissues are similar in purity, protein composition, and tumor antigenicity