PXD068540

PXD068540 is an original dataset announced via ProteomeXchange.

Title	Mouse TAPBPR functions as an MHC-I peptide exchange catalyst
Description	Human TAPBPR is known to function as a Major Histocompatibility Complex class I (MHC-I) peptide exchange catalyst which shapes the peptide repertoire presented to immune cells. Never14theless, investigations characterizing TAPBPR from other species are limited. Here, we characterize mouse TAPBPR, exploring its association partners in mouse cell lines and comparing its function to human TAPBPR. We find that mouse TAPBPR binds MHC-I and calnexin, with a notably sustained interaction with H2-Db compared to H2-Kb. We reveal mouse TAPBPR mediates peptide exchange on both H2-Db and H2-Kb, restricting the peptide repertoire presented on MC-38 cells. Intriguingly, mouse TAPBPR promotes the selection of peptides with a C-terminal methionine on H2-Kb. Together, our findings establish that mouse TAPBPR plays an important role in shaping the MHC-I immunopeptidome by functioning as a peptide editor, like its human counterpart.
HostingRepository	PRIDE
AnnounceDate	2026-03-23
AnnouncementXML	Submission_2026-03-23_08:07:29.473.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louise Boyle
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-19 01:53:44	ID requested
⏵ 1	2026-03-23 08:07:29	announced

Dataset with its publication pending

submitter keyword: H-2Kb,mouse TAPBPR, peptide exchange, H-2Db, MHC-I, calnexin

Louise Boyle
contact affiliation	Professor of Molecular Immunology, Wellcome Trust Senior Research Fellow Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge UK, CB2 1QP
contact email	lhb22@cam.ac.uk
lab head
Louise Boyle
contact affiliation	University of Cambridge
contact email	lhb22@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068540
     1. Label: PRIDE project
     2. Name: Mouse TAPBPR functions as an MHC-I peptide exchange catalyst