PXD068485

PXD068485 is an original dataset announced via ProteomeXchange.

Title	Supplementary Dataset S5. AvrRpm1 ADP-ribosylates MAMI when expressed in N. benthamiana.
Description	During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein AvrRpm1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to identify AvrRpm1-mediated ADP-ribosylation sites on Arabidopsis AT5G54110 (MAMI) by co-expression in Nicotiana benthamiana. The affinity enrichment was performed using the enhanced Af1521 Macro domain versus a G42E mutant variant as control.
HostingRepository	PRIDE
AnnounceDate	2025-11-17
AnnouncementXML	Submission_2025-11-16_22:52:07.310.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lennart Wirthmueller
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-18 06:07:01	ID requested
⏵ 1	2025-11-16 22:52:07	announced

10.1002/PLD3.70115;

submitter keyword: LC-MSMS, Arabidopsis thaliana, affinity purification,plant, Pseudomonas syringae, ADP-ribosylation, AvrRpm1, plant pathogen, type 3 effector

Lennart Wirthmueller
contact affiliation	Leibniz Institute of Plant Biochemistry, Department Biochemistry of Plant Interactions, Weinberg 3, 06120 Halle (Saale), Germany
contact email	lennart.wirthmueller@ipb-halle.de
lab head
Lennart Wirthmueller
contact affiliation	Leibniz Institute of Plant Biochemistry Dept. Biochemistry of Plant Interactions Weinberg 3 06120 Halle (Saale)
contact email	lennart.wirthmueller@ipb-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068485
     1. Label: PRIDE project
     2. Name: Supplementary Dataset S5. AvrRpm1 ADP-ribosylates MAMI when expressed in N. benthamiana.