PXD068468

PXD068468 is an original dataset announced via ProteomeXchange.

Title	High-purity isolation and chemical composition of stabilisomes from thermophilic cyanobacteria
Description	Thermophilic cyanobacteria are not only fundamental models for investigating thermotolerance mechanisms but also a valuable reservoir for the development of novel thermophilic bioresources. Progress in both of these areas, however, is contingent upon the precise characterization of their key subcellular organelles, such as the intracellular polyphosphate-rich granules which we term stabilisomes. The lack of a high-purity, structure-preserving isolation method has been a major technical bottleneck hindering such research. This study describes a high-fidelity, structure-preserving purification strategy, boosting the granule-to-protein yield by over 10,000-fold compared with conventional methods. The high fidelity of this method is confirmed by the retention of complex proteomic (937 proteins) and metabolomic (1,076 metabolites) signatures. Building on this, subsequent quantitative analysis across cyanobacteria at 7 hot spring sampling sites revealed a conserved core chemical composition dominated by polyphosphate (21.1%-36.4%), proteins (9.9%-20.0%), amino acids (1.6%-7.4%), and lipids (12.0%-21.0%), which nevertheless showed significant variabilitiy in the abundance of these components across species. This work establishes a robust methodological framework for isolating prokaryotic stress-responsive organelles, lays the foundation for mechanistic investigations into microbial adaptation, and serves as a prerequisite for exploring the biomedical applications of these organelles.
HostingRepository	PRIDE
AnnounceDate	2025-09-18
AnnouncementXML	Submission_2025-09-17_20:39:57.934.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chenyu Wang
SpeciesList	scientific name: Synechococcus sp. M1; NCBI TaxID: 1307174;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-17 20:03:58	ID requested
⏵ 1	2025-09-17 20:39:58	announced

Dataset with its publication pending

submitter keyword: Thermophilic cyanobacteria

polyphosphate

stabilisomes

purification methods

Chenyu, Wang
contact affiliation	No. 163 Xianlin Avenue, Qixia District, Nanjing City, Jiangsu Province
contact email	18851732535@163.com
lab head
Chenyu Wang
contact affiliation	Nanjing University
contact email	18851732535@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD068468

PRIDE project URI

• PRIDE
  1. PXD068468
     1. Label: PRIDE project
     2. Name: High-purity isolation and chemical composition of stabilisomes from thermophilic cyanobacteria