PXD068462

PXD068462 is an original dataset announced via ProteomeXchange.

Title	Identification of RNA chaperones by gradient profiling in the Lyme disease spirochete, Borrelia burgdorferi
Description	RNA-binding proteins (RBPs) play key roles in regulating gene expression in bacteria. However, relatively few RBPs have been discovered and characterized in Borrelia (Borreliella) burgdorferi, the causative agent of Lyme disease. We utilized gradient profiling to identify putative RBPs that co-sediment with small RNAs (sRNAs) and nascent mRNAs. We employed in vitro and in vivo assays to characterize the RNA chaperone activities of several proteins we identified in the gradient profiling. The previously hypothetical proteins BB0749, BB0713 and BB0796, as well as the chemotaxis-related protein CheY2 and the flagella-associated protein FlgV displayed dramatic RNA annealing and/or strand displacement activity. Moreover, in vivo Co-IP assays demonstrated BB0749 binds RNA in B. burgdorferi.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:24:16.324.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD068462
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Ebmeier
SpeciesList	scientific name: Borreliella burgdorferi; NCBI TaxID: NEWT:139;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-17 11:08:46	ID requested
⏵ 1	2026-06-28 16:24:17	announced

10.6019/PXD068462;

10.1080/15476286.2026.2684395;

Van Gundy T, Schneider C, Ebmeier CC, Chambers GZ, Cramer NA, Chambers KM, Drecktrah D, Hyde JA, Skare JT, Marconi RT, Samuels DS, Lybecker MC, . RNA Biol, 23(1):1-23(2026) [pubmed]

submitter keyword: Lyme disease, Borrelia burgdorferi, RNA chaperone, RNA-binding protein

Meghan Lybecker
contact affiliation	Center for Disease Control
contact email	oru7@cdc.gov
lab head
Christopher Ebmeier
contact affiliation	University of Colorado Boulder
contact email	christopher.ebmeier@colorado.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD068462

PRIDE project URI

• PRIDE
  1. PXD068462
     1. Label: PRIDE project
     2. Name: Identification of RNA chaperones by gradient profiling in the Lyme disease spirochete, Borrelia burgdorferi