PXD068454

PXD068454 is an original dataset announced via ProteomeXchange.

Title	Molecular evaluation of different enrichment methods of extracellular vesicles from bio-banked serum from healthy subjects
Description	Extracellular vesicles (EVs) from human body fluids are important tools for biomarker discovery and for exploring the mechanisms underlying different pathologies, including infectious diseases. The translation of EVs research into clinical practice is however hindered by the variability in EV pre-clinical investigations, thus standardisation of analytical procedures and reporting policies is essential. Human serum is a key biological matrix for biomarker discovery and commonly stored within biobanks. Here we evaluated the impact of different enrichment strategies on EVs downstream analyses. EVs were obtained from 250μl of bio-banked serum using ultracentrifugation (UC), size exclusion chromatography-based methods (ExoSpin - ES, qEV1-35nm – i35 and qEV1-70nm – i70) or ExoRNeasy (ER), prior to morphological evaluation, enumeration, surface phenotyping and multi-‘omics characterisation. All methods enriched adequate amounts of small EVs bearing on their surface tetraspanins, although at different concentrations. The most efficient method for proteomics analyses was qEV1-70nm, followed by ES, which however was more susceptible to contamination from serum proteins. EV-miRNA cargo was efficiently characterised in UC-, ES- and ER-EVs, with the latter providing the highest coverage of vesicular miRNA. Our results support the possibility of using bio-banked serum for EV-based research and further highlight the importance of selecting EV enrichment methods, since they shapes both miRNA and protein landscape.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_16:12:31.913.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcello Manfredi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-17 05:25:27	ID requested
⏵ 1	2026-02-01 16:12:33	announced

10.3390/ijms27020892;

Deiana M, Vezzelli E, Mazzi C, Lavezzari D, Manfredi M, Moretta F, Piubelli C, Gobbi FG, Tiberti N, Molecular Evaluation of Different Enrichment Methods for Extracellular Vesicles from Healthy Subjects' Biobanked Serum. Int J Mol Sci, 27(2):(2026) [pubmed]

submitter keyword: Reproducibility, Mass Spectrometry,Extracellular vesicles, Proteomics

Marcello Manfredi
contact affiliation	University of Piemonte Orientale
contact email	marcello.manfredi@med.uniupo.it
lab head
Marcello Manfredi
contact affiliation	University of Piemonte Orientale
contact email	marcello.manfredi@med.uniupo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD068454

PRIDE project URI

• PRIDE
  1. PXD068454
     1. Label: PRIDE project
     2. Name: Molecular evaluation of different enrichment methods of extracellular vesicles from bio-banked serum from healthy subjects