PXD068440

PXD068440 is an original dataset announced via ProteomeXchange.

Title	Integrated Peptidomic/Proteomic identification of novel cognition-enhancing peptides from Cerebroprotein Hydrolysate that protect against glutamate-induced excitotoxicity in PC12 cells
Description	Cerebroprotein Hydrolysate (CH) is a nutrient extracted from porcine brain tissue that is rich in bioactive peptides. This study first demonstrated that CH exhibits significant learning and memory enhancement activity through behavioral experiments in APP/PS1 mice. However, the specific components and mechanisms of action remained unclear. Integrated peptidomics and bioinformatics screening identified the peptide KAPGLLPR as a highly promising candidate for cognitive enhancement. KAPGLLPR demonstrated significant protective effects against glutamate-induced excitotoxicity in PC12 cells, evidenced by increased cell viability and reduced intracellular oxidative stress. Further proteomic analysis revealed that peptide KAPGLLPR significantly affected the key proteins such as GPX4 (antioxidant), Usp15, and Casp9 (pro-apoptotic). This study not only identifies a novel cognition-enhancing peptide from CH but also elucidates its neuroprotective mechanism, providing a new strategy for exploring and developing CH-based therapeutics.
HostingRepository	iProX
AnnounceDate	2025-09-14
AnnouncementXML	Submission_2025-09-17_00:52:45.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bingkun Yang
SpeciesList	scientific name: Pigmentiphaga sp. D-2; NCBI TaxID: 1002116; scientific name: Mus ; NCBI TaxID: 10088; scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-17 00:51:58	ID requested
⏵ 1	2025-09-17 00:52:45	announced

Dataset with its publication pending

submitter keyword: Cerebroprotein Hydrolysate, Peptidomics, Bioactive Peptides, Oxidative Stress, Alzheimer's Disease

Dezhi Kong
contact affiliation	Hebei Medical University
contact email	kongdezhi@hebmu.edu.cn
lab head
Bingkun Yang
contact affiliation	Hebei Medical University
contact email	yangbingkun0106@163.com
dataset submitter

iProX dataset URI