⮝ Full datasets listing
PXD068428
PXD068428 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterisation of the Tilapia Lake Virus proteome |
| Description | We investigated expression and localisation of all predicted proteins of Tilapia Lake Virus (TiLV) using in vitro translation, cell expression systems, and mass spectrometry, confirming major polypeptides from each segment and discovering an additional 11th protein, S9-F3, from an alternative reading frame. GFP-tagged constructs showed S2 and S10 localised mainly to the nucleus, while S1, S3, S5, S8 and S9-F3 were cytoplasmic, with S5 and S6 forming perinuclear foci. Bioinformatics and inhibitor assays revealed CRM1-dependent nuclear export of S9-F3. Evolutionary analyses indicated selective pressure on S9 and S9-F3 and identified an S9-F3 homologue in a TiLV-like guppy virus. These results reveal a novel protein and regulated nuclear export mechanism, offering new insights into TiLV biology and host interactions. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-01-08 |
| AnnouncementXML | Submission_2026-01-08_00:31:28.732.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dominic Kurian |
| SpeciesList | scientific name: Tilapia; NCBI TaxID: 8126; |
| ModificationList | NeuGc |
| Instrument | timsTOF fleX |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-16 14:44:41 | ID requested | |
| ⏵ 1 | 2026-01-08 00:31:29 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Tilapia, TiLV, nuclear export, accessory protein, CRM1, Leptomycin B, DatasetType:Proteomics |
Contact List
| Paul Digard | |
|---|---|
| contact affiliation | The Roslin Institute, University of Edinburgh |
| contact email | Paul.Digard@roslin.ed.ac.uk |
| lab head | |
| Dominic Kurian | |
| contact affiliation | University of Edinburgh |
| contact email | dominic.kurian@roslin.ed.ac.uk |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099204/ |
