PXD068427

PXD068427 is an original dataset announced via ProteomeXchange.

Title	Molecular architecture of the tRNA ligase complex
Description	In human cells, a subset of tRNA-encoding genes contain introns. These are removed by a non-canonical splicing pathway in which the tRNA splicing endonuclease complex catalyzes intron excision and the resulting exons are subsequently ligated by the tRNA-ligase complex (tRNA-LC). The tRNA-LC is a hetero-pentameric protein assembly consisting of Ashwin, CGI-99, FAM98B, the DEAD-box helicase DDX1 and the catalytic subunit RTCB/HSPC117. We show that the paralogous proteins FAM98A and FAM98C underpin the assembly of compositionally distinct RTCB-containing complexes that lack Ashwin and may have distinct cellular functions.
HostingRepository	PRIDE
AnnounceDate	2026-08-04
AnnouncementXML	Submission_2026-08-04_04:53:11.366.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Pfleiderer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-16 14:03:54	ID requested
⏵ 1	2026-08-04 04:53:11	announced

Dataset with its publication pending

submitter keyword: Human, tRNA, RNA, Protein complex

Martin Jinek
contact affiliation	University of Zurich, Department of Biochemistry
contact email	jinek@bioc.uzh.ch
lab head
Moritz Pfleiderer
contact affiliation	University of Zurich
contact email	mo.pfleiderer@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD068427

PRIDE project URI

• PRIDE
  1. PXD068427
     1. Label: PRIDE project
     2. Name: Molecular architecture of the tRNA ligase complex