PXD068425

PXD068425 is an original dataset announced via ProteomeXchange.

Title	Deciphering Photosynthetic Protein Networks: A Crosslinking-MS Strategy for Studying Functional Thylakoid Membranes
Description	Photosynthesis relies on a dynamic network of protein-protein interactions that adapt to fluctuating light conditions. We present an XL-MS pipeline applied directly to functional thylakoid membranes from Arabidopsis thaliana and Spinacia oleracea. By combining PhoX crosslinker with the amphiphilic salt TMPAC, we captured reproducible structural insights into photosynthetic protein complexes while maintaining photosynthetic activity during crosslinking. The approach enables detection of hundreds of unique crosslinks within three days, expanding thylakoid protein interaction maps, and providing structural models of known and novel complexes. This study demonstrates the feasibility of probing functional photosynthetic membranes with XL-MS, paving the way for integrative analyses of dynamic organellar networks.
HostingRepository	PRIDE
AnnounceDate	2026-05-14
AnnouncementXML	Submission_2026-05-14_08:01:09.323.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pascal Albanese
SpeciesList	scientific name: Spinacia sp.; NCBI TaxID: NEWT:29723; scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-16 13:11:36	ID requested
⏵ 1	2026-05-14 08:01:09	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana,Crosslinking-MS, Thylakoid membranes, Photosynthesis, Protein-protein interactions, Structural proteomics, Spinacia oleracea

Pascal Albanese
contact affiliation	Light, Photosynthesis & Metabolism (LPM) & Exploring the Dynamics of Proteomes (EDyP) IRIG, CEA-Grenoble 17 rue des Martyrs, 38000 Grenoble, France
contact email	pascal.albanese@cea.fr
lab head
Pascal Albanese
contact affiliation	Light, Photosynthesis & Metabolism (LPM) & Exploring the Dynamics of Proteomes (EDyP) IRIG, CEA-Grenoble 17 rue des Martyrs, 38000 Grenoble, France
contact email	pascal.albanese@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD068425

PRIDE project URI

• PRIDE
  1. PXD068425
     1. Label: PRIDE project
     2. Name: Deciphering Photosynthetic Protein Networks: A Crosslinking-MS Strategy for Studying Functional Thylakoid Membranes