PXD068376

PXD068376 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Structural basis of gate dynamics at the outer membrane lipopolysaccharide holo-translocon
Description	Gram-negative bacteria resist many harmful chemicals, protected by an asymmetric outer membrane (OM) containing lipopolysaccharide (LPS) in its external leaflet. Leaflet-selective assembly of LPS is an essential, yet mechanistically elusive process. This reaction is mediated by the OM LPS core translocon, composed of the transmembrane protein LptD and its cognate lipoprotein LptE. Here, we characterize two additional translocon subunits, the lipoproteins LptM and YedD, uncovering their impact on LptD conformational dynamics. This dataset includes native MS allowing the identification of YedD, after gas-phase dissociation from the LptDE/LptDEM complexes (complex-down MS). We also include here bottom-up proteomics data after trypsin digestion from SDS-PAGE gel bands, confirming the nature of YedD in the purified complex.
HostingRepository	PRIDE
AnnounceDate	2025-12-01
AnnouncementXML	Submission_2025-11-30_16:11:20.724.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julien Marcoux
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Synapt MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-15 04:51:07	ID requested
⏵ 1	2025-11-30 16:11:21	announced

Publication List

10.1038/s41467-025-65370-2;
Chen H, Siroy A, Morales V, Gurvi, č D, Quentin Y, Balor S, Abuta'a YA, Marteau M, Froment C, Caumont-Sarcos A, Marcoux J, Stansfeld PJ, Fronzes R, Ieva R, Structural basis of lipopolysaccharide assembly by the outer membrane translocon holo-complex. Nat Commun, 16(1):10404(2025) [pubmed]

10.1038/s41467-025-65370-2;

Chen H, Siroy A, Morales V, Gurvi, č D, Quentin Y, Balor S, Abuta'a YA, Marteau M, Froment C, Caumont-Sarcos A, Marcoux J, Stansfeld PJ, Fronzes R, Ieva R, Structural basis of lipopolysaccharide assembly by the outer membrane translocon holo-complex. Nat Commun, 16(1):10404(2025) [pubmed]

Keyword List

submitter keyword: LPS translocon,native MS, gel digestion

submitter keyword: LPS translocon,native MS, gel digestion

Contact List

Julien Marcoux
contact affiliation	ProteoToul, Institute of Pharmacology and Structural Biology (IPBS), CNRS, Toulouse
contact email	julien.marcoux@ipbs.fr
lab head
Julien Marcoux
contact affiliation	IPBS
contact email	julien.marcoux@ipbs.fr
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD068376
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD068376

PRIDE project URI

Repository Record List

[ + ]