PXD068373

PXD068373 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Heat shock induces silent ribosomes and reorganizes mRNA turnover
Description	mRNAs associate with single or multiple ribosomes these ribosomal assemblies monosomes and polysomes translate the mRNAs before degradation. The impact of heat stress on this mRNA turnover remains unclear. We show that in heat-shocked yeast cells, the proportion of monosomes increases without a corresponding rise in the number of associated mRNAs. Consequently, most monosomes are devoid of mRNAs and silent, lacking translational initiation factors and proteins facilitating posttranslational folding. Such silent monosomes also appear under other stress conditions, with proportions varying according to stress type, suggesting they represent a general feature of cellular adaptation. In parallel with the induction of silent ribosomes, elevated temperatures reduce the overall rate of mRNA ribosome association with few exceptions. Notably, heat-shock promotes the ribosomal association of transcripts encoding heatshock proteins, without extension of the halflives of these mRNAs. These mechanisms dynamically reorganize mRNA turnover to prioritize translation of heat-shock proteins over other proteins.
HostingRepository	MassIVE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-15_04:47:25.175.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex Schmidt
SpeciesList	scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-15 04:21:38	ID requested
⏵ 1	2025-09-15 04:47:25	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: mRNA turnover, heatshock, DIA, DatasetType:Proteomics

submitter keyword: mRNA turnover, heatshock, DIA, DatasetType:Proteomics

Contact List

Alexander Schmidt
contact affiliation	Biozentrum, Universtiy of Basel, 4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alex Schmidt
contact affiliation	University of Basel
contact email	alex.schmidt@unibas.ch
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099165/