PXD068370

PXD068370 is an original dataset announced via ProteomeXchange.

Title	The seed mitochondrial proteome of Lupinus albus provides insight into energy metabolism during germination
Description	Despite their essential role in fueling the onset of metabolism, the composition and functional state of seed mitochondria are still a matter of debate. Using white lupin (Lupinus albus) as a model, we provide a comprehensive proteomic characterization of seed mitochondria in a legume with protein-rich reserves. Highly enriched mitochondrial fractions isolated from quiescent seeds revealed fully assembled oxidative phosphorylation (OXPHOS) complexes and supercomplexes, demonstrating that mitochondria are preconfigured for immediate energy production upon imbibition. Quantitative proteomics identified 1,162 mitochondrial proteins and highlighted marked tissue-specific differences compared to leaves, including distinct transporter profiles and a dehydrogenase complement supporting amino acid and fatty acid catabolism. Early germination was accompanied by remodeling of coenzyme metabolism and transport capacity, while core respiratory complexes remained stable. Notably, ~12% of the proteome consisted of uncharacterized proteins, many of which displayed dynamic changes during early germination, suggesting yet undiscovered, potentially legume-specific mitochondrial functions.
HostingRepository	PRIDE
AnnounceDate	2025-09-19
AnnouncementXML	Submission_2025-09-18_23:39:47.949.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Björn Heinemann
SpeciesList	scientific name: Lupinus albus; NCBI TaxID: 3870;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-15 03:02:29	ID requested
⏵ 1	2025-09-18 23:39:48	announced

Dataset with its publication pending

submitter keyword: proteomics, shotgun proteomics, timsTOF-HT,Lupinus albus, seed, mitochondria, energy metabolism, germination, quantitative proteomics, DDA

Prof. Dr. Tatjana Hildebrandt
contact affiliation	University of Cologne CEPLAS/ Institute for Plant Sciences Plant Metabolic Biochemistry Department Zülpicher Straße 47a D-50674 Cologne Germany
contact email	t.hildebrandt@uni-koeln.de
lab head
Björn Heinemann
contact affiliation	Universität zu Köln Abteilung Pflanzenstoffwechselbiochemie Institut für Pflanzenwissenschaften
contact email	bjoern.heinemann@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD068370

PRIDE project URI

• PRIDE
  1. PXD068370
     1. Label: PRIDE project
     2. Name: The seed mitochondrial proteome of Lupinus albus provides insight into energy metabolism during germination