PXD068367

PXD068367 is an original dataset announced via ProteomeXchange.

Title	Nitrated products formed on α-synuclein are preferentially incorporated into oligomers but excluded from fibrils: a mechanism for accumulation of neurotoxic species
Description	Post-translational modifications (PTMs) such as nitration of Tyr (Y) residues and di-tyrosine (DT) formation are known to impact the aggregation behavior of α-synuclein (α-syn), a protein closely linked to Parkinson’s disease. Using tetranitromethane (TNM) as a model nitrating agent, we systematically investigated the chemical modifications of α-syn and their consequences for aggregation. Mass spectrometry analysis revealed selective nitration of all four Tyr residues, with Y39 and Y125 being most susceptible. DT crosslinks were also observed, primarily involving Y39, but were disfavored at higher TNM concentrations, indicating competition between nitration and crosslinking pathways.
HostingRepository	PRIDE
AnnounceDate	2026-06-19
AnnouncementXML	Submission_2026-06-19_12:20:05.080.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luke Gamon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	nitrated L-tyrosine
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-15 02:30:58	ID requested
⏵ 1	2026-06-19 12:20:05	announced

10.1016/J.BBAPAP.2025.141118;

submitter keyword: tetranitromethane

3-nitrotyrosine

di-tyrosine

crosslinks

fibril formation

Parkinson’s

neurodegeneration

Per Hägglund
contact affiliation	Department of Biomedical Sciences, University of Copenhagen, Denmark
contact email	hagglundperm@gmail.com
lab head
Luke Gamon
contact affiliation	The University of Copenhagen
contact email	lgamon@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD068367

PRIDE project URI

• PRIDE
  1. PXD068367
     1. Label: PRIDE project
     2. Name: Nitrated products formed on α-synuclein are preferentially incorporated into oligomers but excluded from fibrils: a mechanism for accumulation of neurotoxic species