PXD068330

PXD068330 is an original dataset announced via ProteomeXchange.

Title	Prenylomic analysis in AML-3 using tellurium-functionalized isoprenoid probes and OSTAC reaction
Description	This experiment was designed to identify prenylated proteins in AML-3 cells using tellurium-functionalized isoprenoid probes in combination with the oxidation-controlled tellurophene–alkyne cycloaddition (OSTAC) reaction. AML-3 cells were treated with the tellurium probe, which can be metabolically incorporated into prenylated proteins by endogenous prenyltransferases. After labeling, lysates were subjected to the OSTAC reaction using BCN-biotin sulfone, allowing selective biotinylation of probe-modified proteins. These biotinylated proteins were subsequently captured on streptavidin beads and digested on-bead. The resulting peptides were fractionated and analyzed by LC–MS/MS/MS. This workflow enabled the selective enrichment and identification of prenylated proteins from complex AML-3 lysates.
HostingRepository	PRIDE
AnnounceDate	2026-02-12
AnnouncementXML	Submission_2026-02-11_21:41:58.647.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuanzhe Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	farnesylated residue; TMT6plex-126 reporter+balance reagent acylated residue; biotinylated residue; geranylgeranylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-12 11:11:46	ID requested
⏵ 1	2026-02-11 21:41:59	announced

10.1021/JACS.5C19824;

submitter keyword: Prenylation, TMT, Tellurium, Leukemia

Mark Distefano
contact affiliation	Department of Chemistry, University of Minnesota, Minneapolis, Minnesota, US
contact email	diste001@umn.edu
lab head
Yuanzhe Chen
contact affiliation	University of Minnesota
contact email	chen6980@umn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD068330

PRIDE project URI

• PRIDE
  1. PXD068330
     1. Label: PRIDE project
     2. Name: Prenylomic analysis in AML-3 using tellurium-functionalized isoprenoid probes and OSTAC reaction