PXD068315 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Running title: Highly pure isolation of small extracellular vesicles from bovine plasma Title: Technical note: Efficient enrichment of plasma-derived extracellular vesicles from small volumes of bovine blood |
| Description | This study presents a scalable method for isolating small extracellular vesicles (sEVs) according to MISEV (minimal information for studies of extracellular vesicles) guidelines from 4 mL of bovine plasma while achieving a high purity suitable for proteomics analysis. The protocol combines ultracentrifugation (UC), size-exclusion chromatography (SEC), and ultrafiltration (UF) to minimize contamination by lipoproteins and free proteins. The purified sEVs were characterized through transmission electron microscopy (TEM), tunable resistive pulse sensing (TRPS), dynamic light scattering (DLS), and flow cytometry (FC), assessing particle size, morphology, and the presence of membrane markers such as CD9. The purity assessed by the particle-to-protein ratio (4.8 × 10⁸ particles/µg in average) was improved (× 10) compared to the previous protocol. Mass spectrometry (LC-MS/MS) and Parallel Reaction Monitoring (PRM) identified 417 proteins, 93% of which are referenced in sEVs databases. Specific markers of sEVs have been identified including cytosolic (HSPA8, SDCBP, ACT, TUB, and GAPDH) and membrane (CD9 and CD81) Markers. Gene Ontology (GO) analysis revealed an enrichment of sEVs proteins. This study demonstrates that this sEVs enrichment protocol is well-suited for small plasma volumes. These results pave the way for future studies of biomarker research on animal health and performance. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-17 |
| AnnouncementXML | Submission_2025-11-16_17:53:11.236.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD068315 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Didier VIALA |
| SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-12 05:25:17 | ID requested | |
| ⏵ 1 | 2025-11-16 17:53:12 | announced | |
Publication List
| 10.6019/PXD068315; |
| Prieur V, Isabelle CM, Arnaud D, Liam BC, Jean-Christophe D, Isabelle M, Sylvain L, Didier V, Philip C, C, é, line B, Muriel B, Efficient enrichment of plasma-derived extracellular vesicles from small volumes of bovine blood. J Anim Sci, 103():(2025) [pubmed] |
| 10.1093/jas/skaf354; |
Keyword List
| submitter keyword: Biomarker discovery, methodology optimization, phenotypic expressions, proteomics, vesicle-associated proteins, lipoprotein removal |
Contact List
| BONNET Muriel |
|---|
| contact affiliation | Research Director at INRAE, Clermont Auvergne University, VetAgro Sup, Herbivores Joint Research Unit (UMRH) FRANCE |
| contact email | muriel.bonnet@inrae.fr |
| lab head | |
| Didier VIALA |
|---|
| contact affiliation | INRAE |
| contact email | didier.viala@inrae.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068315
- Label: PRIDE project
- Name: Running title: Highly pure isolation of small extracellular vesicles from bovine plasma Title: Technical note: Efficient enrichment of plasma-derived extracellular vesicles from small volumes of bovine blood
