PXD068300

PXD068300 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicles-mediated transfer of let-7b/7c promotes the proliferation of transition-state spermatogonia in neonatal mouse testis
Description	The self-renewal and differentiation of spermatogonial stem cells (SSCs) play essential roles in spermatogenesis. Extracellular vesicle (EV) is a universal strategy for intercellular communications in stem cell niches. However, the involvement of EVs in regulating the SSCs remains largely unknown. In this study, we have revealed the role of testis EVs isolated from postnatal day 7 (PND7) neonatal mouse testis in guiding spermatogonia into a transit-amplifying state with increased proliferation while retaining their differentiation potential. We profiled the repertoires of proteins and small RNAs by proteomic and small RNA transcriptomic analyses, respectively. We further showed that the EVs secreted by undifferentiated spermatogonia and the Sertoli cell lines, but not from more differentiated germ cell lines, conveyed let-7b/7c miRNA cargoes to spermatogonia, which mediates the effect of EVs on spermatognial transit amplification. Together, this study has deciphered an important intercellular communication within the spermatogonial niche mediated by let-7b/7c cargoes of EVs, providing a new insight into the regulation of SSCs and spermatogenesis
HostingRepository	iProX
AnnounceDate	2025-09-12
AnnouncementXML	Submission_2026-01-04_00:12:02.073.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting Zheng
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-11 19:09:32	ID requested
⏵ 1	2026-01-04 00:12:03	announced

Zheng T, Choy KHK, Chan SY, Zheng M, Luo X, Chen H, Xie T, Fok EKL, Extracellular-vesicle-mediated transfer of let-7b/7c promotes the proliferation of transition-state spermatogonia in neonatal mouse testis. Stem Cell Reports, 20(11):102681(2025) [pubmed]

submitter keyword: spermatogonial stem cells, spermatogonia, extracellular vesicle, let-7 miRNA, intercellular communication

Kin Lam FOK Ellis
contact affiliation	The Chinese University of Hong Kong
contact email	ellisfok@cuhk.edu.hk
lab head
Ting Zheng
contact affiliation	Shenzhen Third People's Hospital
contact email	zhengtt@link.cuhk.edu.hk
dataset submitter

iProX dataset URI