PXD068296

PXD068296 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of protein interactions with engineered CRISPR activators
Description	Current protein engineering methods are inadequate to explore the combinatorial potential offered by nature’s vast repertoire of protein domains – limiting our ability to create optimal synthetic tools. To overcome this barrier, we develop an approach to create and test thousands of chimeric proteins and employ it to probe an expansive combinatorial landscape of over 15,000 multi-domain CRISPR activators. Our findings indicate that many activators produce substantial cellular toxicity, often unrelated to their capacity to regulate gene expression. We also explore the biochemical features of activation domains and determine how their combinatorial interactions shape activator behavior. Finally, we identify two potent CRISPR activators, MHV and MMH, and demonstrate their enhanced activity across diverse targets and cell types compared to the gold-standard MCP activator, synergistic activation mediator (SAM)
HostingRepository	PRIDE
AnnounceDate	2025-10-17
AnnouncementXML	Submission_2025-10-16_18:31:18.454.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richa Tiwari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-11 14:40:52	ID requested
⏵ 1	2025-10-16 18:31:18	announced

Dataset with its publication pending

submitter keyword: CRISPR activators, APMS

Nevan J Krogan
contact affiliation	1. Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, CA, USA. 2. Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.
contact email	Nevan.Krogan@ucsf.edu
lab head
Richa Tiwari
contact affiliation	Specialist
contact email	richa.tiwari@ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD068296

PRIDE project URI

• PRIDE
  1. PXD068296
     1. Label: PRIDE project
     2. Name: Mass spectrometry analysis of protein interactions with engineered CRISPR activators