PXD068290

PXD068290 is an original dataset announced via ProteomeXchange.

Title	Unraveling Performance: Proteomic Insights into Photo-Crosslinkable Biopolymer Properties
Description	Rational design of gelatin methacryloyl (GelMA) hydrogels remains limited by insufficient control over their molecular architecture, despite their broad use in bioactive scaffolds. Especially for applications involving tissue regeneration, a delicate balance is needed between mechanical stability and the ability of cells to remodel their microenvironment. In this study, we present a novel integrative workflow combining bottom-up proteomic analysis, 3D molecular modeling, and structural characterization to elucidate how chemical and physical modifications influence GelMA network formation. A 3D model was built from proteomics data identifying site-specific methacryloyl modifications on gelatin-derived COL1α1 and COL1α2 chains. Increasing methacrylic anhydride equivalents (2.5 vs. 0.5 eq) raised the degree of substitution (GelMA 96 vs. GelMA 34) and the frequency of modification, while preserving the number of modified lysines and arginines. Circular dichroism and X-ray diffraction revealed that highly substituted GelMA showed increased β-strand formation at the expense of helices and turns. Upon photo-crosslinking, networks with different crosslink densities were created and, for the first time, covalent crosslinks were identified and localized using proteomic analysis. This structural mapping demonstrated diverse crosslinking motifs and highlighted how enzyme specificity influences detection. The resulting insights connect GelMA chemistry to its crosslinking behavior and secondary structure, enabling more precise tuning of material properties. This work contributes a foundational strategy for decoding bioactive hydrogel networks and supports improved design of GelMA scaffolds for applications such as osteogenesis.
HostingRepository	PRIDE
AnnounceDate	2026-01-13
AnnouncementXML	Submission_2026-01-13_03:00:22.384.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BRAY FABRICE
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	monohydroxylated residue; protein modification categorized by chemical process; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-11 06:15:02	ID requested
⏵ 1	2026-01-13 03:00:22	announced

10.1016/J.APMT.2025.103072;

submitter keyword: gelatin methacryloyl, proteomics, photo-crosslinking, hydrogel, collagen

fabrice bray
contact affiliation	​​Laboratoire MSAP UAR 3290 CNRS Bâtiment C4 Avenue Paul Langevin Cité Scientifique 59655 Villeneuve D'ascq 03 20 33 71 12 https://msap-lab.fr/
contact email	fabrice.bray@univ-lille.fr
lab head
BRAY FABRICE
contact affiliation	MSAP USR 3290
contact email	fabrice.bray@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD068290

PRIDE project URI

• PRIDE
  1. PXD068290
     1. Label: PRIDE project
     2. Name: Unraveling Performance: Proteomic Insights into Photo-Crosslinkable Biopolymer Properties