PXD068274 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In vivo crosslinking, cell fractionation, immunoprecipitation and MS identification of proteins crosslinked to E. coli GapA-3xFLAG |
| Description | Our BATCH assay suggested that glycolytic enzymes could associate into complexes of varied stoichiometry and potentially interact with proteins outside of glycolysis, moreover, PfkA, FbaA and GapA were the top interacting proteins, showing strong interactions with both the PTS and PNPase. We constructed individual E. coli strains expressing chromosomally-encoded 3xFLAG-tagged variants of these enzymes (i.e., E. coli pfkA-3xFLAG, E. coli fbaA-3xFLAG and E. coli gapA-3xFLAG) and monitored the expression of the that the 3xFLAG-tagged proteins by western blotting. GapA was the most abundant of the proteins, followed by FbaA and PfkA, the E. coli gapA-3xFLAG strain was subsequently used for in vivo crosslinking and immunoprecipitation (CLIP-MS). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-16 |
| AnnouncementXML | Submission_2026-07-16_03:51:09.662.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD068274 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Sue Lin-Chao |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-10 23:18:37 | ID requested | |
| ⏵ 1 | 2026-07-16 03:51:10 | announced | |
Publication List
Keyword List
| submitter keyword: Escherichia, MS, immunoprecipitation, crosslinking |
Contact List
| Sue Lin-Chao |
|---|
| contact affiliation | Institute of Molecular Biology, Academia Sinica |
| contact email | mbsue@gate.sinica.edu.tw |
| lab head | |
| Sue Lin-Chao |
|---|
| contact affiliation | Intitute of Molecular Biology, Academia Sinica |
| contact email | mbsue@gate.sinica.edu.tw |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD068274 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068274
- Label: PRIDE project
- Name: In vivo crosslinking, cell fractionation, immunoprecipitation and MS identification of proteins crosslinked to E. coli GapA-3xFLAG
