PXD068214

PXD068214 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	PHF19 drives the formation of PRC2 clusters to enhance motility in TNBC cells
Description	Polycomb Repressive Complex 2 (PRC2) is a key regulator of transcriptional repression and chromatin organization, essential in development and disease. While its enzymatic activity is well characterized, the factors governing PRC2 subnuclear organization, particularly in cancer cells, are largely unknown. Here, we integrate in situ subcellular proteomics, high-resolution imaging, and functional genomics to investigate PRC2 compartmentalization in triple-negative breast cancer (TNBC) cells. We identify PHF19, a sub-stoichiometric PRC2 accessory subunit as upregulated in TNBC, and central to the formation of endogenous, micron-scale nuclear PRC2 clusters. These structures act as spatial hubs that stabilize local PRC2 occupancy and reinforce H3K27me3 macro-domain organization. Mechanistically, an intrinsically disordered region (IDR) in PHF19 is required for clustering and for promoting TNBC cell motility. Our findings uncover a non-enzymatic layer of PRC2 regulation, where local PRC2 compartmentalization through accessory subunits, directly influences cellular behavior, with implications for disease and development.
HostingRepository	MassIVE
AnnounceDate	2025-09-09
AnnouncementXML	Submission_2025-09-09_17:20:08.153.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jung-chi Liao
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Deamidated; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-08 23:09:32	ID requested
⏵ 1	2025-09-09 17:20:08	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Polycomb, EZH2, PCL3, Chromatin organization, H3K27me3, Biomolecular condensates, IDR, Triple-negative Breast Cancer, Cell migration, Optoproteomics, DatasetType:Proteomics

submitter keyword: Polycomb, EZH2, PCL3, Chromatin organization, H3K27me3, Biomolecular condensates, IDR, Triple-negative Breast Cancer, Cell migration, Optoproteomics, DatasetType:Proteomics

Contact List

Cristiana Lungu
contact affiliation	Institute of Cell Biology and Immunology, University of Stuttgart; Stuttgart Research Center Systems Biology, University of Stuttgart
contact email	cristiana.lungu@izi.uni-stuttgart.de
lab head
Jung-chi Liao
contact affiliation	SYNCELL INC
contact email	liaojc@syncell.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099095/