PXD068210

PXD068210 is an original dataset announced via ProteomeXchange.

Title	PROTEOMIC AND FUNCTIONAL ANALYSIS OF ALPACA (VICUGNA PACOS) SPERM QUALITY FOLLOWING IN VITROCAPACITATION WITH FOLLICULAR AND OVIDUCTAL FLUIDS
Description	Assisted reproductive technologies, such as in vitro fertilization, remain inefficient in camelids, largely due to gaps in understanding the molecular interactions that regulate sperm capacitation. Fertilization requires not only viable spermatozoa but also precise modulation of capacitation by the peri-ovulatory microenvironment, including follicular fluid (FF) and oviductal fluid (OF). In this study, spermatozoa were incubated in Fert-TALP medium supplemented with FF or OF, and both functional outcomes and proteomic remodeling were assessed. FF was collected from preovulatory follicles (7–9 mm) and OF from the corresponding ipsilateral oviducts, thereby reflecting the in vivo environment encountered by sperm in the female reproductive tract following mating. Incubation with FF enhanced motility, viability, and acrosome responsiveness, and was associated with a proteomic shift involving ~12% of proteins. These included factors implicated in zona pellucida binding (LYPD4, PGK1, ANXA2, TCP1 complex members) and galactose metabolism (MAOA, AKR1B1, GLA, HK1). The enriched processes included glycolysis/gluconeogenesis, cytoskeletal reorganization, and protein maturation, all consistent with sperm capacitation. By contrast, sperm incubated with OF showed underrepresentation of capacitation-related pathways, including the proteasome complex, fibrous sheath, and TCA cycle. Moreover, OF proteome revealed decapacitation-associated factors such as PEBP1 and PAFAH1B3, which likely stabilize membranes and delay premature capacitation. Together, these findings demonstrate complementary yet contrasting roles of FF and OF in modulating sperm physiology: FF acting as a capacitating medium, and OF providing a stabilizing environment. This work delivers the first partial proteome of capacitated alpaca sperm together with matched reproductive fluids, providing mechanistic insights with direct implications for improving assisted reproduction in camelids.
HostingRepository	PRIDE
AnnounceDate	2025-10-27
AnnouncementXML	Submission_2025-10-27_15:31:54.832.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Argañaraz
SpeciesList	scientific name: Vicugna pacos; NCBI TaxID: NEWT:30538;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-08 21:47:13	ID requested
⏵ 1	2025-10-27 15:31:55	announced

10.3389/FVETS.2025.1702095;

submitter keyword: oviductal fluid, follicular fluid, sperm quality,south amercan camelids, spermatozoa

Martin E. Argañaraz
contact affiliation	Instituto Superior de Investigaciones Biológicas (INSIBIO), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Tucumán (CONICET-UNT), Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, Argentina
contact email	martin.arganaraz@fbqf.unt.edu.ar
lab head
Martin Argañaraz
contact affiliation	Instituto Superior de Investigaciones Biológicas (INSIBIO)/CONICET-UNT
contact email	martin.arganaraz@fbqf.unt.edu.ar
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068210
     1. Label: PRIDE project
     2. Name: PROTEOMIC AND FUNCTIONAL ANALYSIS OF ALPACA (VICUGNA PACOS) SPERM QUALITY FOLLOWING IN VITROCAPACITATION WITH FOLLICULAR AND OVIDUCTAL FLUIDS