PXD068208

PXD068208 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Notch signaling regulates the secretion of pro-metastatic factors in extracellular vesicles in liposarcoma
Description	Notch signaling is an emerging regulator of liposarcoma (LPS) but its role in mediating communication with the tumor microenvironment (TME) is unclear. Here, we investigate how Notch activation (NICD overexpression) alters proteomes of LPS-derived extracellular vesicles (EVs). We used quantitative mass spectrometry to profile EV proteome in multiple contexts: cultured LPS cells, LPS tumor, circulating EVs of LPS-bearing mice and human LPS samples. We found that Notch signaling increases the secretion of EV proteins that favors tumor progression and metastasis but suppresses immune responses in murine LPS cells. Overlapping murine and human LPS data identifies 18 proteins that are increased in LPS EVs of both species, including endotrophin as a biomarker of LPS. Functional analysis supports a role of LPS EVs in regulating gene expression and behaviors of endothelial cells in TME. Together, these data demonstrate that in addition to its known function in driving tumorigenesis, Notch signaling also regulates TME through EV secretion.
HostingRepository	jPOST
AnnounceDate	2025-09-09
AnnouncementXML	Submission_2025-09-08_21:19:15.759.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi-Kai Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-08 21:18:29	ID requested
⏵ 1	2025-09-08 21:19:16	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Liposarcoma, Tumor microenvironment, Extracellular vesicles, Proteomics

submitter keyword: Liposarcoma, Tumor microenvironment, Extracellular vesicles, Proteomics

Contact List

Shihuan Kuang
lab head
Yi-Kai Liu
contact affiliation	Purdue University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004061/