PXD068197

PXD068197 is an original dataset announced via ProteomeXchange.

Title	Optimizing Mass Spectrometry Methods to Uncover M. tuberculosis Biomarkers in Extracellular Vesicles
Description	Individuals exposed to Mtb can experience a range of outcomes: early bacterial clearance, latent Mtb infection (LTBI), asymptomatic TB (bacteriologically confirmed or not), or symptomatic TB (bacteriologically confirmed or clinically diagnosed). Current tools cannot reliably predict or distinguish these stages. Previous studies have identified Mtb peptides in extracellular vesicles (EVs) from TB patients’ serum, highlighting their potential as biomarkers. We aimed to identify Mtb proteins and peptides in serum EVs as potential biomarkers across TB stages, with a focus on differentiating asymptomatic individuals. We evaluated data-independent acquisition (DIA) mass spectrometry with different data analysis strategies to identify and differentially quantify Mtb peptides. We analyzed serum samples from healthy, HIV-negative South African adult volunteers enrolled in a TB risk study. Samples were categorized as prevalent TB (diagnosed within 30 days of baseline), incident TB (collected at baseline from participants who later developed TB), activated TB (collected at diagnosis, during follow-up, from incident cases), or controls (collected at baseline from participants who did not develop TB within the 15 months of the study).
HostingRepository	PRIDE
AnnounceDate	2026-03-03
AnnouncementXML	Submission_2026-03-03_14:43:03.502.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luisa Nieto Ramirez
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: NEWT:83332;
ModificationList	monohydroxylated residue
Instrument	timsTOF fleX MALDI-2

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-08 18:17:55	ID requested
⏵ 1	2026-03-03 14:43:04	announced

10.1093/INFDIS/JIAG086;

submitter keyword: asymptomatic, proteomics, subclinical TB, discovery,Serum, mycobacterium

Karen Dobos
contact affiliation	Professor, Microbiology, Immunology, and Pathology Mycobacteria Research Laboratories Scientific Director, Rocky Mountain Regional Biocontainment Laboratory Editor-in-chief, BMC Mycobacteria
contact email	Karen.Dobos@ColoState.edu
lab head
Luisa Nieto Ramirez
contact affiliation	Colorado State University
contact email	luisam@colostate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD068197

PRIDE project URI

• PRIDE
  1. PXD068197
     1. Label: PRIDE project
     2. Name: Optimizing Mass Spectrometry Methods to Uncover M. tuberculosis Biomarkers in Extracellular Vesicles