PXD068196

PXD068196 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteome-wide characterization of FAS2 genomic elements swap
Description	Protein synthesis is a highly regulated process that ensures the efficient assembly of protein complexes. Co-translational interactions are crucial to ensure the timely formation of functional protein complexes. One factor that can facilitate complex assembly is the spatial organization of mRNA within the cell. In this study we investigate the spatial distribution of mRNAs encoding for protein complex subunits in Saccharomyces cerevisiae, focusing on determining the co-localization state of these mRNAs and its impact on protein assembly and functionality. By swapping cis-regulatory elements upstream and downstream of the coding sequence we identified patterns that influence mRNA co-localization. We further quantified the resulting changes in global protein abundance in the swapped strains using LC-MS.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-09_03:41:09.962.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rawad Hanna
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-08 14:55:35	ID requested
⏵ 1	2026-02-09 03:41:10	announced

Publication List

10.1016/j.jmb.2025.169546;
Shhade Q, Estrada ME, Hanna R, Makhzumy M, Bar-Yosef H, Kramer G, Bukau B, Shiber A, Distinct Cis-acting Elements Combinatorically Mediate Co-localization of mRNAs Encoding for Co-translational Interactors in Cytoplasmic Clusters in S. cerevisiae. J Mol Biol, 438(2):169546(2026) [pubmed]

10.1016/j.jmb.2025.169546;

Shhade Q, Estrada ME, Hanna R, Makhzumy M, Bar-Yosef H, Kramer G, Bukau B, Shiber A, Distinct Cis-acting Elements Combinatorically Mediate Co-localization of mRNAs Encoding for Co-translational Interactors in Cytoplasmic Clusters in S. cerevisiae. J Mol Biol, 438(2):169546(2026) [pubmed]

Keyword List

submitter keyword: Complex, Yeast, FAS2,Co-translational

submitter keyword: Complex, Yeast, FAS2,Co-translational

Contact List

Dr. Ayala Shiber
contact affiliation	Faculty of Biology, Technion, Israel
contact email	ayalashiber@technion.ac.il
lab head
Rawad Hanna
contact affiliation	Postdoc- Technion Israel Institute of Technology
contact email	rawad.hanna.10@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD068196
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD068196

PRIDE project URI

Repository Record List

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