⮝ Full datasets listing
PXD068187
PXD068187 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Stu1-3xFLAG immunoprecipitation from benomyl treatment, Cdc20-AID treatment, and DMSO control |
| Description | Log-phase Stu1-3xFLAG Cdc20-AID OsTIR1 cells were grown in 3 conditions: 0.1% DMSO (control, asynchronous), 30 ug/mL benomyl with 0.1% DMSO (metaphase arrest), or 1 mM auxin (indole-3-acetic acid) with 0.1% DMSO (metaphase arrest) for 2.5 hours at 23C prior to harvesting. Harvested yeast were washed in water supplemented with 0.2 mM PMSF, pelleted, and resuspended in lysis buffer (Buffer H 0.15): 25 mM HEPES pH 8.0, 150 mM KCl, 2 mM MgCl2, 0.1 mM EDTA pH 8.0, 0.1% NP-40, 15% glycerol, supplemented with protease and phosphatase inhibitors. Yeast pellets were frozen in liquid nitrogen and lysed using a Freezer Mill (SPEX, Metuchen NJ). Lysate was clarified via ultracentrifugation at 24,000 RPM (98,000 x g) for 90 minutes and the protein layer was extracted with a syringe. This extract was incubated with magnetic a-M3DK antibody (targeting Stu1-3xFLAG) conjugated Dynabeads (GenScript) for 3 hours at 4C with rotation, washed in lysis buffer 5 times, washed 2 times in 50 mM Tris pH 8.3, 75 mM KCl, 1 mM EGTA, and eluted with 0.2% RapiGest (Waters Corporation, Milford MA) in 50 mM ammonium bicarbonate for mass spectrometry processing. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-01-12 |
| AnnouncementXML | Submission_2026-01-12_15:42:39.622.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Fred Hutch Proteomics Core |
| SpeciesList | scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932; |
| ModificationList | Phospho |
| Instrument | Orbitrap Fusion ETD |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-08 09:45:56 | ID requested | |
| ⏵ 1 | 2026-01-12 15:42:40 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Saccharomyces cerevisiae, Stu1, benomyl, microtubule, kinetochore, DatasetType:Proteomics |
Contact List
| Susan Biggins | |
|---|---|
| contact affiliation | Fred Hutchinson Cancer Center |
| contact email | sbiggins@fredhutch.org |
| lab head | |
| Fred Hutch Proteomics Core | |
| contact affiliation | Fred Hutchinson Cancer Research Center |
| contact email | pgafken@fredhutch.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099081/ |
