PXD068177

PXD068177 is an original dataset announced via ProteomeXchange.

Title	Kingella kingae KK03 Type IV Pili
Description	Type IV Pili (T4P) are dynamic surface appendages that mediate adherence, motility, and DNA uptake in Kingella kingae, an important pediatric pathogen that causes osteoarticular infections, bacteremia, and endocarditis. While the major pilin subunit in K. kingae T4P is well characterized, the contribution of minor pilins to T4P structure and function remains unknown. Here, we used proteomics, molecular genetics, biochemical analyses, and structural modeling to identify and characterize all eight minor pilins in K. kingae. We identified a conserved operon of core minor pilin genes encoding FimT, PilV, PilW, PilX, and PilE that promotes surface piliation, adherence to epithelial cells, twitching motility, and natural transformation. Deletion of the fimTpilVWXE locus phenocopied loss of the PilC1 and PilC2 adhesins, and AlphaFold modeling combined with bacterial two-hybrid analysis suggested that FimT, PilV, PilW, and PilX form a complex at the pilus tip. The PilA2, ComP, and KK03_01180 minor pilins were dispensable for adherence and motility but promoted natural transformation and formed protein-protein interactions with the major pilin, suggesting that these proteins are incorporated throughout the pilus shaft. These findings support a new model for the architecture of the K. kingae type IV pilus, with distinct minor pilins localizing to different sites on the pilus fiber and mediating specialized functions essential for virulence.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-06_10:52:00.613.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD068177
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Taylor Yount
SpeciesList	scientific name: Kingella kingae PYKK081; NCBI TaxID: NEWT:1150867; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-08 04:03:24	ID requested
⏵ 1	2025-10-06 10:52:01	announced

10.6019/PXD068177;

submitter keyword: Type IV Pili, Bacteria

Joseph St. Geme
contact affiliation	Children's Hospital of Philadelphia
contact email	stgemeiiij@chop.edu
lab head
Taylor Yount
contact affiliation	University of Pennsylvania
contact email	tyount@pennmedicine.upenn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD068177

PRIDE project URI

• PRIDE
  1. PXD068177
     1. Label: PRIDE project
     2. Name: Kingella kingae KK03 Type IV Pili