PXD068173

PXD068173 is an original dataset announced via ProteomeXchange.

Title	Acupuncture at ST36 for alleviating Central Sensitization in Rheumatoid Arthritis: A Study on the Regulatory Mechanism of Lgals3bp Based on Quantitative Proteomics
Description	Rheumatoid arthritis (RA), as a chronic autoimmune disease, is characterized by persistent joint pain and functional impairment. Recent studies have confirmed that pain, as the most prominent clinical symptom in RA patients, is closely related to multidimensional pathological processes, including the combined effects of peripheral nerve sensitization and central nervous system sensitization. This study investigates the regulatory mechanisms of acupuncture intervention on central sensitization processes using a mouse model of adjuvant-induced arthritis (AIA) induced by Complete Freund's Adjuvant (CFA). The research employs quantitative proteomics analysis, Western blot, immunofluorescence , and real-time quantitative PCR to monitor changes in key signaling molecules within spinal astrocytes. Experimental data indicate that acupuncture treatment significantly alleviates CFA-induced hyperalgesia, with its mechanism closely related to the downregulation of Lgals3bp protein expression in spinal astrocytes. Further molecular studies reveal that acupuncture effectively reduces the transcription levels of pro-inflammatory mediators TNF-α, IL-6, and CXCL5 by inhibiting the phosphorylation and activation of the NF-κB signaling pathway. These findings suggest that acupuncture may alleviate central sensitization by modulating the neuroimmune network mediated by glial cells. This study not only provides new experimental evidence for the central analgesic mechanism of acupuncture in treating RA but also elucidates the crucial role of the Lgals3bp/NF-κB signaling axis in neuroimmune regulation, offering important theoretical basis for clinical practice.
HostingRepository	iProX
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-08_01:57:20.199.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Feiyang Li
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-08 01:56:27	ID requested
⏵ 1	2025-09-08 01:57:20	announced

Dataset with its publication pending

submitter keyword: Acupuncture, Rheumatoid Arthritis, Central Sensitization, Spinal Cord, Lgals3bp

Zhiyu Zheng
contact affiliation	School of Acupuncture & Moxibustion and Tuina, Tianjin University of Traditional Chinese Medicine, Tianjin City, People’s Republic of China
contact email	zhiyu16622761988@163.com
lab head
Feiyang Li
contact affiliation	School of Acupuncture & Moxibustion and Tuina, Tianjin University of Traditional Chinese Medicine
contact email	1766950085@qq.com
dataset submitter

iProX dataset URI