PXD068166

PXD068166 is an original dataset announced via ProteomeXchange.

Title	Decoding the Regulatory Networks of Proteus mirabilis Under Succinic Acid Stress: A Multi-Omics Approach
Description	Proteus mirabilis, a key catheter-associated urinary tract infection pathogen that forms antibiotic-resistant crystalline biofilms, was shown via multi-omics analyses to be multimodally inhibited by succinic acid. At 15 mM, succinic acid reduced the bacterium’s growth by 70% and biofilm formation by 50%. Metabolomics revealed dysregulation in tryptophan/arginine metabolism, nucleotide biosynthesis, and the tricarboxylic acid cycle; transcriptomics showed downregulated ribosomal genes, oxidative phosphorylation, and efflux pumps plus upregulated arginine transport; and proteomics demonstrated suppressed T6SS virulence factors and iron acquisition proteins. Mechanistically, succinic acid is proposed to reduce K6 acetylation of the histone-like nucleoid structuring protein, enhancing its oligomerization to repress T6SS genes and inhibit biofilm formation. By targeting metabolism, virulence, and stress adaptation, succinic acid circumvents single-target resistance, offering a strategy to combat multidrug-resistant P. mirabilis via biofilm disruption and pathogenicity suppression.The uploaded data comprise two omics datasets:Acetyl-proteomics: Folders prefixed with XB10893DPAc (treatment: H1–H3; control: T1–T3)；Proteomics: Folders prefixed with XB10893DA (treatment: H1–H3; control: T1–T3).
HostingRepository	iProX
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-08_01:40:13.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiancheng Li
SpeciesList	scientific name: Proteus mirabilis; NCBI TaxID: 584;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-08 01:35:21	ID requested
⏵ 1	2025-09-08 01:40:13	announced

Dataset with its publication pending

submitter keyword: Multi-omics

Succinic acid

Biofilm formation

Proteus mirabilis

Xiancheng Li
contact affiliation	The Second Affiliated Hospital of Dalian Medical University
contact email	lxc2620@163.com
lab head
Xiancheng Li
contact affiliation	The Second Affiliated Hospital of Dalian Medical University
contact email	lxc2620@163.com
dataset submitter

iProX dataset URI