PXD068137

PXD068137 is an original dataset announced via ProteomeXchange.

Title	BOLL-containing Aggregates Mediate the Translational Regulation During Human Oogenesis
Description	Humanoocyte meiosis utilizes a specialized translational control strategy tocoordinate meiotic progression, mediated through dynamic regulation of mRNAstores. While germ cell-specific RNA-binding proteins (RBPs) are known toorchestrate this post-transcriptional program, the mechanistic basis ofRBP-mediated cell fate specification remains elusive. Here, we demonstrate that 000000000000000BOLL, a Deleted in Azoospermia (DAZ) family protein, forms protein aggregatesduring meiotic prophase to drive translational reprogramming in humanoogenesis. We determined that BOLL enhance translation efficiency of cell cycleregulators, as demonstrated by integrative translatome-transcriptome analysiscombined with RNA immunoprecipitation sequencing. We also revealed the functionalinteraction network of BOLL with coretranslation machinery components through its conserved DAZ containing domain. Crucially, we identified the SDS-resistantprotein aggregates as BOLL structural signature in human oocyte-like cells,demonstrated by semi-denaturing electrophoretic analysis. These finding delineate a protein aggregate-mediatedregulatory paradigm where BOLL aggregates exert spatiotemporal control overcell cycle related genes during the meiotic prophase of human oogenesis.
HostingRepository	PRIDE
AnnounceDate	2026-04-03
AnnouncementXML	Submission_2026-04-03_00:15:50.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ying Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-05 20:18:30	ID requested
⏵ 1	2026-04-03 00:15:50	announced

10.1111/CPR.70181;

submitter keyword: translational regulation, protein aggregate,human BOLL protein, oogenesis

Kehkooi Kee
contact affiliation	The State Key Laboratory for Complex, Severe, and Rare Diseases; SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, 100084, China.
contact email	kkee@tsinghua.edu.cn
lab head
Ying Li
contact affiliation	Fuzhou University
contact email	liying.2018@tsinghua.org.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD068137

PRIDE project URI

• PRIDE
  1. PXD068137
     1. Label: PRIDE project
     2. Name: BOLL-containing Aggregates Mediate the Translational Regulation During Human Oogenesis