PXD068096

PXD068096 is an original dataset announced via ProteomeXchange.

Title	N6-methylations of adenine are recognized explicitly by proteins using adjacent aromatic amino acid side chains
Description	N6-methyladenosine (m6A) is the most abundant nucleotide modification in eukaryotic mRNA. Changes in the levels of m6A are implicated in many human diseases and conditions such as various types of cancer, diabetes, neurological diseases, male infertility, and obesity. However, molecular mechanisms for m6A recognition by various readers are not well. We used phage display to identify key sequence requirements of methyl readers for m6A recognition. The RNA sequence as well as the secondary structure in which m6A is located was shown to influence its recognition by methyl readers. Further, adjacent aromatic residues are involved in m6A recognition, possibly in a manner similar to that of YTH-domain containing m6A readers. RNA pulldown assays identified many candidates that could possibly be novel m6A readers. Among them, hnRNPA1 was identified as a promising m6A reader. Alignment of hnRNAPA1 along with the known m6A readers of hnRNP family of proteins against the most abundant peptide selected from phage display, shows the conservation of adjacent aromatic residues. These aromatic residues are located in between the regions known to be important for RNA binding by hnRNPA1. We believe these aromatic residues and m6A increase the specificity of RNA recognition by hnRNPA1. This information on the evolutionary pressure exerted by m6A on its readers can be used to discover novel m6A readers
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_16:36:37.518.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD068096
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Belinda Willard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-04 17:32:55	ID requested
⏵ 1	2026-02-01 16:36:38	announced

10.3390/biom16010097;

10.6019/PXD068096;

Mollah A, Rauff R, Abedeera S, Ekanayake C, Thalagaha Mudiyanselage C, To M, Piontkivska H, Abeysirigunawardena S, A Modification in RNA. Biomolecules, 16(1):(2026) [pubmed]

submitter keyword: methyl reader,epitranscriptomics, ITC, m6A, phage display

Sanjaya Abeysirigunawardena (Abey), Ph.D.
contact affiliation	Associate Professor Department of Chemistry and Biochemistry 126 Integrated Sciences Building Kent State University
contact email	sabeysir@kent.edu
lab head
Belinda Willard
contact affiliation	Cleveland Clinic
contact email	willarb@ccf.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD068096

PRIDE project URI

• PRIDE
  1. PXD068096
     1. Label: PRIDE project
     2. Name: N6-methylations of adenine are recognized explicitly by proteins using adjacent aromatic amino acid side chains