PXD068090

PXD068090 is an original dataset announced via ProteomeXchange.

Title	Affinity purification of the Legionella effector SidL in HEK293T cells
Description	Legionella pneumophila is an intracellular bacterial pathogen that encodes almost 300 effector proteins, many of which target various aspects of its eukaryotic host. While the physiological roles of many effectors are not understood, the focused study of individual effectors provides insight into their often novel and unique biochemistries. Here, we characterize the function of one effector, SidL, who was reported to inhibit host translation. We find that SidL blocks the nutrient-responsive translation regulator mTORC1, suggesting that translation inhibition is a downstream consequence of SidL activity. We use computational analyses that classify SidL as a member of a phosphotransferase and oxidoreductase superfamily, including a recently described protein-modifying adenylyltransferase; however, SidL does not adenylate proteins. Instead we use in vitro biochemistry to discover that SidL adenylates the glycolysis intermediate 3-phosphoglycerate (3PG), producing the novel metabolite 2-AMP-3PG. In cells, SidL disrupts glycolysis with a notable loss of 3PG and downstream intermediates. We propose that SidL expression consumes 3PG, causing metabolic perturbations that inactivate mTORC1, consequentially inhibiting translation. To our knowledge, this is the first report of a bacterial effector uniquely modifying an intermediate in a conserved metabolic pathway and suggests a novel mechanism by which bacteria modulate host metabolism.
HostingRepository	PRIDE
AnnounceDate	2026-07-24
AnnouncementXML	Submission_2026-07-24_08:25:35.314.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joshua Black
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-04 13:53:13	ID requested
⏵ 1	2026-07-24 08:25:35	announced

10.1016/J.MOLCEL.2026.07.007;

submitter keyword: Legionella pneumophila, actin,SidL

Rachel Green
contact affiliation	Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Howard Hughes Medical Institute, Chevy Chase, MD, USA
contact email	ragreen@jhmi.edu
lab head
Joshua Black
contact affiliation	Johns Hopkins University School of Medicine
contact email	jjblack91@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068090
     1. Label: PRIDE project
     2. Name: Affinity purification of the Legionella effector SidL in HEK293T cells