⮝ Full datasets listing
PXD068081
PXD068081 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Early cardiomyocyte dysfunction from anthracycline exposure is driven by conserved RNA protein co expression networks |
| Description | This study uses human induced pluripotent stem cell-derived cardiomyocytes iPSC-CMs from six individuals treated with sublethal, clinically relevant doses of three ACs Doxorubicin, Epirubicin, Daunorubicin and a related TOP2B-inhibiting drug Mitoxantrone to model chemotherapeutic associated cardiotoxicity 3 and 24 hours post treatment. Peptide mixtures were analyzed by nanoflow liquid chromatography-tandem mass spectrometry using a nano LC chromatography system UltiMate 3000 RSLCnano, Dionex, coupled on-line to a Thermo Orbitrap Eclipse mass spectrometer through a nanospray ion source. Instrument performance was verified by analyzing a standard six protein mix digest before the sample set run, between each experimental block and at the end of the experiment. Protein batches were block randomized by individual, where batch contained sequentially randomized samples from each individual. One protein mix digest of the individual block was run before and after all randomized samples. A protein mix digest comprising all 60 experimental samples at the end of each run was also measured. The protein mix data files were analyzed to confirm that instrument performance remained consistent throughout the experiment. In total 60 experimental samples were treated with one drug at one timepoint per individual. 17 samples were pooled by batch. 9 pooled samples consisted of total pool samples. This lead to a total of 88 samples analyzed further in downstream analysis. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-11 |
| AnnouncementXML | Submission_2025-12-11_15:16:39.105.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sayan |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | unknown modification |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-04 09:47:04 | ID requested | |
| ⏵ 1 | 2025-12-11 15:16:39 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Anthracycline, Cardiomyocyte, Cardiotoxicity, DatasetType:Proteomics |
Contact List
| Michelle C. Ward | |
|---|---|
| contact affiliation | University of Texas Medical Branch at Galveston |
| contact email | miward@utmb.edu |
| lab head | |
| Sayan | |
| contact affiliation | Paul |
| contact email | saypaul@utmb.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099030/ |
