PXD068063 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics on pancreatic ductal organoids |
| Description | Primary human pancreatic ductal organoids (HPDO) have emerged as a model to study pancreas biology and model disease like pancreatitis and pancreatic cancer. Yet, donor material availability, genetic variability and a lack of extensive benchmarking to healthy and disease pancreas limits the range of applications. To address this gap, we established porcine pancreatic ductal organoids (PPDO) as a system from a reliable, genetically defined and easily obtainable source to model pancreatic ductal/progenitor biology. We benchmarked PPDO to HPDO and primary porcine pancreas using single-cell RNA sequencing (scRNA-Seq). We observed no overt phenotypic differences in PPDO derived from distinct developmental stages using extensive proteomics profiling, with a WNT/basal cell signaling enriched population characterizing PPDO. PPDO exhibited differentiation potential towards mature ductal cells and limited potential towards endocrine lineages. We used PPDO as a chemical screening platform to assess the safety of FDA-approved drugs and showed conserved toxicity of statins and α-adrenergic receptor inhibitors between PPDO and HPDO cultures. Overall, our results highlight the PPDO as a model for mammalian duct/progenitor applications. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-10 |
| AnnouncementXML | Submission_2025-11-10_03:17:00.098.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Christine von Toerne |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF; timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-04 05:58:04 | ID requested | |
| ⏵ 1 | 2025-11-10 03:17:00 | announced | |
Publication List
Keyword List
| submitter keyword: Pancreatic ductal organoids |
| porcine pancreas |
| chemical screen |
| cross-species comparison |
| scRNA-Seq profile |
| proteome profile; |
Contact List
| Heiko Lickert |
|---|
| contact affiliation | Institute of Diabetes and Regeneration Research, Helmholtz Munich, Neuherberg, Germany |
| contact email | heiko.lickert@helmholtz-munich.de |
| lab head | |
| Christine von Toerne |
|---|
| contact affiliation | Helmholtz Zentrum München |
| contact email | vontoerne@helmholtz-muenchen.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD068063 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068063
- Label: PRIDE project
- Name: Proteomics on pancreatic ductal organoids
