PXD068034

PXD068034 is an original dataset announced via ProteomeXchange.

Title	POMC neuron AMPKα1 ablation attenuates obesity via BAT activation
Description	Proopiomelanocortin (POMC) neurons in the arcuate nucleus of the hypothalamus (ARC) play a critical role in energy homeostasis. 5’-adenosine monophosphate-activated protein kinase (AMPK) is a serine/threonine kinase, which acts as a main energy sensor in the cell. The heterotrimeric AMPK results from the combination of a catalytic α subunit (α1, α2) with two regulatory subunits, β (β1, β2) and γ (γ1, γ2 and γ3). Hypothalamic AMPK plays a key role in the control of energy balance, but current evidence shows that the precise combination of its heterotrimeric components determines its physiological action. This makes necessary the study of the role of the different subunits in a cell-specific manner. Our findings show that AMPKα1 ablation in POMC neurons has a protective effect over diet-induced obesity because of increased thermogenesis in brown adipose tissue (BAT). At the molecular level, deletion of AMPKα1 in POMC neurons disrupts the ARC phosphoproteome and is associated with an ameliorated endoplasmic reticulum (ER) stress. These findings highlight the complex function of the different AMPK subunits in the hypothalamic regulation of energy balance.
HostingRepository	PRIDE
AnnounceDate	2026-06-30
AnnouncementXML	Submission_2026-06-30_08:26:25.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan A Lopez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-03 08:16:34	ID requested
⏵ 1	2026-06-30 08:26:26	announced

Dataset with its publication pending

submitter keyword: AMPK

BAT

hypothalamus

obesity

POMC

phosphoproteomics

thermogenesis

LC-MS

TMT

Juan A Lopez
contact affiliation	Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, 28029, Spain
contact email	jalopez@cnic.es
lab head
Juan A Lopez
contact affiliation	CNIC
contact email	jalopez@cnic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068034
     1. Label: PRIDE project
     2. Name: POMC neuron AMPKα1 ablation attenuates obesity via BAT activation