PXD068032

PXD068032 is an original dataset announced via ProteomeXchange.

Title	Localisation-dependent variations in articular cartilage ECM: implications for tissue engineering and cartilage repair
Description	Articular cartilage (AC) is a specialised connective tissue covering joint surfaces. It enables smooth movement, distributes mechanical loads, and protects the underlying bone. In re-sponse to loading, AC adapts by modifying both its thickness and composition. AC is or-ganised in different zones, with low cellularity and a high abundance of extracellular ma-trix (ECM). Mechanical overloading or immobilisation can lead to structural changes, po-tentially resulting in osteoarthritis (OA), for which no causal treatment currently exists. However, smaller defects can be treated using chondrocyte/cartilage transplantation or tissue engineering. A better understanding of the molecular composition of AC at different locations is essential to improve such therapeutic approaches. For this purpose, we per-formed a comprehensive analysis of porcine femoral knee cartilage at eight defined ana-tomical sites. Cartilage thickness and proteoglycan (PG) content were analysed histologi-cally, while specific ECM proteins were assessed by proteomics and validated by im-munohistochemistry and Western blot. Significant differences were identified, particularly between medial and lateral compartments, in terms of cartilage thickness, PG abundance, and ECM composition. Some proteins also showed zone-specific localisation patterns. These structural differences likely reflect adaptation to mechanical loading and should be considered to optimise future cartilage repair and tissue engineering strategies.
HostingRepository	PRIDE
AnnounceDate	2025-10-15
AnnouncementXML	Submission_2025-10-15_03:06:36.315.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luisa Marie Schmidt
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-03 05:31:06	ID requested
⏵ 1	2025-10-15 03:06:36	announced

10.3390/IJMS26199331;

submitter keyword: ECM, cartilage,osteoarthritis, pig, proteomcis

Frank Zaucke
contact affiliation	Dr. Rolf M. Schwiete Osteoarthritis Research Unit, Department of Trauma Surgery and Orthopedics, University Hospital, Goethe University, Frankfurt, Germany
contact email	frank.zaucke@ukffm.de
lab head
Luisa Marie Schmidt
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
contact email	luisa.schmidt@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD068032

PRIDE project URI

• PRIDE
  1. PXD068032
     1. Label: PRIDE project
     2. Name: Localisation-dependent variations in articular cartilage ECM: implications for tissue engineering and cartilage repair