PXD068024 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Viral RNA silencing suppressor and DCL4-associated DRB4 protein interaction is modulated by a viral long non-coding RNA |
| Description | The expression of the BNYVV silencing suppressor (VSR) p14 protein, together with the production of a viral non-coding RNA (ncRNA3) affects the long-distance movement on natural host Beta species. Through immunoprecipitation of Flag-p14 and expression of TurboID-p14 coupled to mass spectrometry, we identified several potential p14 cellular partners. These include proteins related to RNA metabolism, proteasome activation, and notably, SGS3 -a key player in the siRNA transitivity pathway- and DRB4, the DCL4 cofactor described in A. thaliana silencing pathways. The interaction between p14 and DRB4 was specifically retrieved with the hypomorphic p14BA2 VSR mutant in nucleoli and was disrupted in the presence of the ncRNA3, a condition that allows the viral mutant to move long distance. Subcellular fractionation of infected tissues revealed that ncRNA3 accumulates in the nucleus thanks to its interaction with p14 while genomic RNA3 remains cytoplasmic. Our results suggest that a specific viral RNA is targeted to nucleus to participate in silencing suppression function through destabilisation of p14-DRB4 complex. Moreover, the putative interaction between p14 and SGS3 provides a mechanistic explanation of the role of this VSR on silencing transitivity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-06_16:19:46.212.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD068024 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Johana Chicher |
| SpeciesList | scientific name: Nicotiana benthamiana; NCBI TaxID: NEWT:4100; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF Pro 2; Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-03 03:51:05 | ID requested | |
| ⏵ 1 | 2026-09-06 16:19:47 | announced | |
Publication List
| 10.6019/PXD068024; |
| Janzam A, Bellott L, Chicher J, Hammann P, Kempff C, Jambois L, Hleibieh K, Michel F, Ziegler-Graff V, Gilmer D, A Viral Long Non-Coding RNA Modulates Viral RNA Silencing Suppressor and DCL4-Associated DRB4 Protein Interaction. Viruses, 18(7):(2026) [pubmed] |
| 10.3390/v18070801; |
Keyword List
| submitter keyword: DRB4, viral RNA silencing suppressor, P14 |
Contact List
| David Gilmer |
|---|
| contact affiliation | Institut de biologie moléculaire des plantes, CNRS UPR2357, Université de Strasbourg, Strasbourg, France |
| contact email | david.gilmer@ibmp-cnrs.unistra.fr |
| lab head | |
| Johana Chicher |
|---|
| contact affiliation | Université de Strasbourg, Plateforme Protéomique Strasbourg-Esplanade, Centre National de la Recherche Scientifique |
| contact email | j.chicher@ibmc-cnrs.unistra.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068024
- Label: PRIDE project
- Name: Viral RNA silencing suppressor and DCL4-associated DRB4 protein interaction is modulated by a viral long non-coding RNA
