PXD068009

PXD068009 is an original dataset announced via ProteomeXchange.

Title	XL-MS of of influenza A NEP/NS2
Description	The influenza nuclear export protein (NEP)/non-structural protein 2 (NS2) is a multifunctional protein, involved in viral ribonucleoprotein (vRNP) export from the nucleus, genome replication enhancement, and the adaption of avian influenza to mammals. Despite the growing attention on the importance of NEP in the influenza lifecycle and in interspecies transmission from avian hosts, the molecular details of how it is able to perform its various roles is still yet to be fully understood. In the interest of generating antiviral proteins and to aid in structural characterization, a panel of Rep proteins were raised against influenza virus A NEPH1N1. When complexed with RepE4 we were able to crystallize full-length NEPH1N1, revealing a folded and monomeric conformation. The N- and C-termini of NEPH1N1 pack together, with the middle linker region resembles a hinge, con-trasting a previous structure where NEP is dimeric and elongated. Together these structures demonstrate the plasticity of NEP, a trait which may potentially aid NEP in binding a diversity of cellular and viral partners. Using isothermal titration calorimetry (ITC) we measured a nanomolar interaction between RepE4 and NEP. Similarly, we found that RepE4 can also bind NEP from hu-man infecting avian H7N9 and bovine originating avian H5N1 influenza viruses. Owing to their high degree of conservation, RepE4 likely has the capacity to interact with NEP from numerous influenza A virus strains. Indeed, this, combined with the nanomolar affinity measured between NEP/RepE4 could be explored further as a broad-range therapeutic strategy and/or a tool in cellulo to understand NEP function at the molecular level.
HostingRepository	PRIDE
AnnounceDate	2026-02-12
AnnouncementXML	Submission_2026-02-12_01:10:24.659.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pascal Albanese
SpeciesList	scientific name: Influenza A virus (A/Philippines/TMC10-144/2010(H1N1)); NCBI TaxID: NEWT:1124765;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-02 15:28:09	ID requested
⏵ 1	2026-02-12 01:10:25	announced

Stelfox AJ, Bessonne M, Bourhis JM, Erba EB, Albanese P, Compte DP, Nevers Q, Urvoas A, Valerio-Lepiniec M, Minard P, Ruigrok RWH, Cr, é, pin T, Delmas B, Ballandras-Colas A, Monomeric Structure of Influenza A Virus NEP/NS2 Obtained With an Artificial Protein Highlights Conformational Plasticity. J Mol Biol, 437(24):169511(2025) [pubmed]

10.1016/j.jmb.2025.169511;

submitter keyword: SDA, influenza, photo-crosslinking, virus, crosslinking

Pascal Albanese
contact affiliation	3 Univ. Grenoble Alpes, CNRS, INRAE, CEA, IRIG-LPCV, 38000 Grenoble, FranceLPCV - to be completed 4. Univ. Grenoble Alpes, CEA, INSERM, UA13 BGE, CNRS, CEA, FR2048, Grenoble, France
contact email	pascal.albanese@cea.fr
lab head
Pascal Albanese
contact affiliation	Light, Photosynthesis & Metabolism (LPM) & Exploring the Dynamics of Proteomes (EDyP) IRIG, CEA-Grenoble 17 rue des Martyrs, 38000 Grenoble, France
contact email	pascal.albanese@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD068009

PRIDE project URI

• PRIDE
  1. PXD068009
     1. Label: PRIDE project
     2. Name: XL-MS of of influenza A NEP/NS2