PXD068008

PXD068008 is an original dataset announced via ProteomeXchange.

Title	Identification of Candidate Interaction Partners of Human Calpain-5 in Neuroblastoma Cells with SWATH-MS Analysis
Description	Few interaction partners of human calpain-5/CAPN5 have been identified or proposed. This project was designed to identify candidate interaction partners of CAPN5 in SH-SY5Y neuroblastoma cells. We utilized sequential window acquisition of all theoretical mass spectra (SWATH‐MS) for proteomic analysis of samples resulting from co-immunoprecipitation with full-length CAPN5-3×FLAG. The bait was catalytically inactive due to a C81A mutation.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_16:20:41.225.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carlos Gartner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-02 14:23:52	ID requested
⏵ 1	2026-02-01 16:20:42	announced

Gal J, Bondada V, Crasta R, Croall DE, Vary CP, Geddes JW, Candidate Interaction Partners of Calpain-5 Suggest Clues to Its Involvement in Neovascular Inflammatory Vitreoretinopathy. Cells, 15(2):(2026) [pubmed]

10.3390/cells15020142;

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Human, CAPN5, neuroblastoma, proteomics, calpain-5, interaction partner, mass spectrometry, immunoprecipitation

Calvin Vary
contact affiliation	Director, MaineHealth Institute for Research, Proteomics and Lipidomics Laboratory
contact email	Calvin.Vary@mainehealth.org
lab head
Carlos Gartner
contact affiliation	MaineHealth Institute for Research
contact email	gusti.gartner@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD068008

PRIDE project URI

• PRIDE
  1. PXD068008
     1. Label: PRIDE project
     2. Name: Identification of Candidate Interaction Partners of Human Calpain-5 in Neuroblastoma Cells with SWATH-MS Analysis