PXD068004

PXD068004 is an original dataset announced via ProteomeXchange.

Title	The proteomic landscape of CTNNB1 mutated low-grade early-stage endometrial carcinomas
Description	Endometrial carcinoma is the most frequent gynecologic malignancy in western countries. In recent years, mutations in CTNNB1 have been associated with worse prognosis in low-risk carcinomas. However, there is a lack of understanding of the proteomic implications of CTNNB1 mutations in this type of tumor. In this study, we performed shotgun proteomics using Formalin Fixed Paraffin Embedded (FFPE) tissue samples of CTNNB1 mutated and wild type low-risk endometrial carcinomas. A publicly available proteomic and transcriptomic database was used to validate results. Differential protein expression and Gene Set Enrichment Analysis revealed dysregulation of pathways associated with cell keratinization, immune response modulation, and intracellular calcium regulation. CTNNB1 mutated tumors showed immune dysregulation at multiple levels including cytokine secretion, cell adhesion, and lymphocyte activation. These results were supported by tissue multiplex immunofluorescence analysis, demonstrating reduced CD8 tumor infiltrating lymphocytes and different immune spatial interaction patterns. Intracellular calcium dysfunction was associated with key transcript dysregulation. We found an increased expression of CAMK2A and ROR2, suggesting a potential role for non-canonical Wnt pathway activation in CTNNB1 mutated tumors.
HostingRepository	PRIDE
AnnounceDate	2025-11-24
AnnouncementXML	Submission_2025-11-23_16:18:56.318.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alberto Pelaez García
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-02 13:24:18	ID requested
⏵ 1	2025-11-23 16:18:57	announced

10.3390/cells14211676;

Lopez-Janeiro A, Brizzi E, Ruz-Caracuel I, Alexandru R, de Andrea C, Berj, ó, n A, Yebenes L, Mendiola M, Heredia-Soto V, Montero-Calle A, Barderas R, de Los Rios V, Redondo A, Pelaez-Garcia A, Hardisson D, Mutated Low-Grade Early-Stage Endometrial Carcinomas. Cells, 14(21):(2025) [pubmed]

submitter keyword: Proteomics

Endometrial Carcinoma

Wnt pathway

Immune microenvironment

Alberto Pelaez-Garcia
contact affiliation	Proteomics Unit (UCCTs), Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain
contact email	alberto.pelaez@isciii.es
lab head
Alberto Pelaez García
contact affiliation	Proteomics Core National Institute of health Carlos III
contact email	albertopelaez84@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD068004

PRIDE project URI

• PRIDE
  1. PXD068004
     1. Label: PRIDE project
     2. Name: The proteomic landscape of CTNNB1 mutated low-grade early-stage endometrial carcinomas