PXD067993

PXD067993 is an original dataset announced via ProteomeXchange.

Title	Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ
Description	Mouse-adapted Staphylococcus aureus strains have become increasingly relevant for infection research due to their ability to better recapitulate clinical infection dynamics in mouse models. However, detailed characterizations to establish an applicable reference strain remains limited. The mouse-adapted CC88 strain JSNZ appears to be an ideal candidate for a reference strain, because CC88 is widely spread among laboratory mice and frequently employed in mouse colonization and infection models. Moreover, JSNZ demonstrates high genetic transformability comparable to that of commonly used laboratory strains. In this study, we present a comprehensive genomic and proteomic characterization of JSNZ. Proteomic profiling was conducted under standard laboratory conditions in TSB and RPMI during exponential and stationary growth using LC-MS/MS. Proteomic profiling of JSNZ indicated broad similarity to common S. aureus reference strains. However, a striking exception was the novel serine protease Jep, which constituted approximately 75% of the exoproteome in stationary TSB cultures. Overall, these findings affirm JSNZ as a robust and genetically tractable model strain for murine S. aureus infection research and contribute a valuable standardized resource to enhance experimental reproducibility and cross-study consistency in the field.
HostingRepository	PRIDE
AnnounceDate	2025-10-28
AnnouncementXML	Submission_2025-10-28_11:05:54.093.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hannes Wolfgramm
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: NEWT:1280; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-02 06:05:52	ID requested
⏵ 1	2025-10-28 11:05:54	announced

10.1016/J.CRMICR.2025.100489;

submitter keyword: JSNZ, serine protease, proteome profiling, reference strain, Jep, mouse infection model,Staphylococcus aureus

Uwe Völker
contact affiliation	Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany
contact email	voelker@uni-greifswald.de
lab head
Hannes Wolfgramm
contact affiliation	University Medicine Greifswald
contact email	hannes.wolfgramm@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD067993

PRIDE project URI

• PRIDE
  1. PXD067993
     1. Label: PRIDE project
     2. Name: Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ