PXD067978
PXD067978 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Toward Minimally Invasive Metabolomics: GC-MS metabolic fingerprints of Dried Blood Microsamples in comparison to Plasma |
| Description | Global untargeted profiles of dried blood microsamples were studied in comparison to conventional plasma and blood samples using Gas Chromatography-Mass Spectrometry (GC-MS). Venous blood from 10 healthy, overnight-fasted individuals was collected and used to produce dried microsamples on Whatman cards, Capitainer and Mitra devices. In parallel paired plasma samples were collected. The metabolite extraction protocol was optimized and methanol was selected as the extraction solvent, while methoximation and trimethylsilylation derivatization was applied. Blood microsampling, mainly from Mitra and Capitainer devices, provided equivalent or greater information than plasma, considering the number and intensity of features, precision, and annotated metabolites intensity. Furthermore, blood microsample metabolic profiles collected from the fingertip of three individuals were compared against paired plasma and blood. Overall, the results suggest that blood microsampling is a viable alternative for untargeted blood metabolomics, providing comparable information. Capillary blood collected using Mitra devices exhibits variations when compared to venous whole blood and plasma, with distinct trends observed across several metabolites. Consequently, further research is necessary to determine the applicability of this approach in specific contexts. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-09-02 |
| AnnouncementXML | Submission_2025-09-02_02:41:41.560.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Daniel Marques de Sa e Silva |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | EVOQ Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-02 02:31:11 | ID requested | |
| ⏵ 1 | 2025-09-02 02:41:41 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Gas Chromatography, Untargeted Metabolomics, Microsampling, DatasetType:Metabolomics |
Contact List
| Helen Gika | |
|---|---|
| contact affiliation | Aristotle University of Thessaloniki |
| contact email | gkikae@auth.gr |
| lab head | |
| Daniel Marques de Sa e Silva | |
| contact affiliation | Aristotle University of Thessaloniki |
| contact email | dmarque@auth.gr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099001/ |
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