PXD067954

PXD067954 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation
Description	HEK293T cells were co-transfected with StrepII-FLAG-tagged DCAF12 and HA-tagged CUL4A, grown for 48 h, harvested, and lysed as described above. In the first step, Strep-TactinXT Superflow resin (IBA Lifesciences, Gottingen, Germany) was used to purify proteins associated with StrepII-FLAG-tagged subsrtrate receptors. In the second step, eluates from the previous step were used for immunoprecipitation of HA-CUL1/4A-associated proteins. Eluates from both steps were analyzed by LC-MS/MS at the Proteomics facility of Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University (BIOCEV) in Vestec, Czech Republic. Further details are provided in Supplementary methods.
HostingRepository	MassIVE
AnnounceDate	2025-09-01
AnnouncementXML	Submission_2025-09-01_09:28:28.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lukas Cermak
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-01 09:26:31	ID requested
⏵ 1	2025-09-01 09:28:29	announced

Publication List

Lidak T, Baloghova N, Korinek V, Sedlacek R, Balounova J, Kasparek P, Cermak L, CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation. Int J Mol Sci, 22(10):(2021) [pubmed]

Lidak T, Baloghova N, Korinek V, Sedlacek R, Balounova J, Kasparek P, Cermak L, CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation. Int J Mol Sci, 22(10):(2021) [pubmed]

Keyword List

submitter keyword: ubiquitin, proteasome, DatasetType:Proteomics

submitter keyword: ubiquitin, proteasome, DatasetType:Proteomics

Contact List

Lukas Cermak
contact affiliation	Institute of Molecular Genetics of the ASCR, v. v. i.
contact email	lukas.cermak@img.cas.cz
lab head
Lukas Cermak
contact affiliation	Institute of Molecular Genetics of the ASCR, v. v. i.
contact email	lukas.cermak@img.cas.cz
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097470/