PXD067920

PXD067920 is an original dataset announced via ProteomeXchange.

Title	Unveiling EMC6 as a Novel Pathogenic Determinant in hepatocellular carcinoma: Orchestration of lipid metabolism through regulation of lipid droplet-associated enzyme HSD17B13
Description	The endoplasmic reticulum (ER) membrane protein complex (EMC) is an ER multiprotein complex that affects a wide range of pathophysiological processes. Recently, the function of EMC6, a subunit of EMC, has been attracting attention for its role in cancers. However, research on EMC6 in the context of hepatocellular carcinoma (HCC) remains unknown. Here, we first observed the decreased EMC6 expression in human HCC tissues, and diminished expression level of EMC6 was associated with poor prognosis of HCC patients. In parallel, the knockdown of EMC6 promoted tumor progression both in HCC cell lines and in tumor-cell bearing nude mice. To delineate the in vivo roles of EMC6, we generated a hepatocyte specific knockout of Emc6 (Emc6f/f;Alb-Cre, named Emc6 LKO) using a floxed Emc6 line. Emc6 LKO mice exhibited progressive liver dysfunction, fibrosis and spontaneous carcinogenesis phenotypes. Significantly lipid metabolic disorder in the Emc6 LKO liver was revealed by combined metabolomic and proteomic analysis. Moreover, drastic elevation of 17β-Hydroxysteroid dehydrogenase type 13 (HSD17B13), a lipid droplet-associated enzyme, was identified to be involved in the process of EMC6-induced lipid metabolic disorder and HCC progression. Inhibition of HSD17B13 by a Pharmalogical inhibitor BI-3231 effectively mitigated EMC6-driven HCC progression in vitro and in vivo. Taken together, these results unveiled a novel regulation mechanism of EMC in HCC progression through lipid metabolism and may provide a new biomarker and therapeutic target for HCC.
HostingRepository	iProX
AnnounceDate	2025-08-31
AnnouncementXML	Submission_2025-09-01_00:17:56.509.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chanyu Xiong
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-31 20:17:51	ID requested
⏵ 1	2025-09-01 00:17:56	announced

Dataset with its publication pending

submitter keyword: EMC6, hepatocellular carcinoma, lipid metabolism, HSD17B13

Yu Zhou
contact affiliation	Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China
contact email	zhouyu422@yahoo.com
lab head
Chanyu Xiong
contact affiliation	Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China
contact email	Chanyuxiong@foxmail.com
dataset submitter

iProX dataset URI