PXD067912

PXD067912 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	P300 proximity labeling in human iPSCs
Description	This dataset presents the results of proximity-dependent biotinylation targeting the transcriptional coactivator p300 in human induced pluripotent stem cells (hiPSCs). We endogenously tagged p300 with the biotin ligase miniTurboID, to enable labeling of proximal proteins and thus proteins locatd at enhancers. hiPSCs were cultured in two different conditions, either StemFit or mTeSR medium, to assess how media composition influences the p300 proximal proteome. Following biotin labeling, proteins were enriched via streptavidin pulldown and analyzed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2026-08-07
AnnouncementXML	Submission_2026-08-07_03:44:46.381.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	S Stelloo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-31 09:33:26	ID requested
⏵ 1	2026-08-07 03:44:47	announced

Publication List

10.1016/j.stemcr.2026.103023;
Alejo-Vinogradova MT, Str, ø, m YE, Lamers LA, Chang YW, Hendriksen MJ, Moosajee M, Vermeulen M, Stelloo S, From stem cells to somites: Revealing genetic and exogenous factors of human embryogenesis. Stem Cell Reports, ():103023(2026) [pubmed]

10.1016/j.stemcr.2026.103023;

Alejo-Vinogradova MT, Str, ø, m YE, Lamers LA, Chang YW, Hendriksen MJ, Moosajee M, Vermeulen M, Stelloo S, From stem cells to somites: Revealing genetic and exogenous factors of human embryogenesis. Stem Cell Reports, ():103023(2026) [pubmed]

Keyword List

submitter keyword: None

submitter keyword: None

Contact List

Prof. Michiel
contact affiliation	Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, 6525 GA Nijmegen, The Netherlands
contact email	michiel.vermeulen@ru.nl
lab head
S Stelloo
contact affiliation	Department of Urology, Radboud University Medical Center (RUMC), Nijmegen, The Netherlands
contact email	stelloo@science.ru.nl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD067912
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD067912

PRIDE project URI

Repository Record List

[ + ]