PXD067906

PXD067906 is an original dataset announced via ProteomeXchange.

Title	Nucleocapsid protein captures DDX5 and RNMT facilitating viral RNA synthesis and viral protein translation for coronavirus replication
Description	Coronaviruses (CoVs) hijack host RNA-binding proteins (RBPs) to facilitate their replication, but the viral proteins and host RBDs that participate in the synthesis of viral RNA and protein are unclear. In this study, we revealed that DEAD-box helicase (DDX5) and staphylococcal nuclease domain-containing protein (SND1) facilitate viral RNA synthesis, and that RNA guanine-7 methyltransferase (RNMT) enhances viral protein translation to promote viral replication via coronaviral subgenomic RNA-protein interactomes. DDX5 and SND1 positively regulate PEDV replication by promoting viral RNA synthesis via the binding of DDX5 to positive-sense viral RNA, whereas SND1 specifically detects negative-sense viral RNA. The interaction of DDX5/SND1 and N/nsp9/nsp12 promotes the formation of replication-transcription complexes for viral RNA synthesis to facilitate viral replication. We found that RNMT captures the host protein translation system to cyclize viral mRNA to assist in viral protein translation to promote viral replication. We also found that DDX5 broadly interacts with the N protein of CoVs and promotes the RNA synthesis of bovine coronavirus and porcine delta-coronavirus to promote viral replication. These results indicate that CoVs use host proteins to assist in the synthesis of viral RNA and protein to facilitate viral replication.
HostingRepository	PRIDE
AnnounceDate	2026-01-21
AnnouncementXML	Submission_2026-01-21_02:06:55.827.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liu Yuchang
SpeciesList	scientific name: Chlorocebus sabaeus; NCBI TaxID: NEWT:60711; scientific name: Sus scrofa (Pig); NCBI TaxID: NEWT:9823;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-30 20:24:52	ID requested
⏵ 1	2026-01-21 02:06:56	announced

10.1128/MBIO.02717-25;

submitter keyword: protein translation, virus-host interaction,coronaviruses, RNA-binding proteins, RNA synthesis

Tongling Shan
contact affiliation	Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
contact email	shantongling@shvri.ac.cn
lab head
Liu Yuchang
contact affiliation	Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
contact email	996689818@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067906
     1. Label: PRIDE project
     2. Name: Nucleocapsid protein captures DDX5 and RNMT facilitating viral RNA synthesis and viral protein translation for coronavirus replication