PXD067896

PXD067896 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Li et al., Xylosyltransferase Bump-and-hole Engineering to Chemically Manipulate Proteoglycans in Mammalian Cells: Decorin glycosylated in-cell with 6AzGlc and clicked with ITag
Description	pgsA-745 cells stably transfected with a plasmid encoding for BH-XT1 were transfected transiently with a plasmid encoding human FLAG-tagged decorin. Cells were fed with 6AzGlc-1-phosphate precursor 1, and supernatant isolated. Decorin was purified, then clicked with ITag-alkyne, digested with trypsin on S-Trap, then analysed by HCD on an Orbitrap Eclipse. Data analysis with Byonic using 6AzGlc-ITag as a custom mod.
HostingRepository	MassIVE
AnnounceDate	2025-10-15
AnnouncementXML	Submission_2025-10-15_05:58:39.707.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Schumann
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	unknown modification
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-30 01:40:40	ID requested
⏵ 1	2025-10-15 05:58:40	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Glycosaminoglycan, chemical proteomics, glycosylation, glycosyltransterase, DatasetType:Proteomics, DatasetType:Other (Glycoproteomics)

submitter keyword: Glycosaminoglycan, chemical proteomics, glycosylation, glycosyltransterase, DatasetType:Proteomics, DatasetType:Other (Glycoproteomics)

Contact List

Benjamin Schumann
contact affiliation	TUD Dresden University of Technology
contact email	benjamin.schumann@tu-dresden.de
lab head
Benjamin Schumann
contact affiliation	TUD Dresden University of Technology
contact email	benjamin.schumann@tu-dresden.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098981/